Department of Otolaryngology, Nishinomiya Municipal Central Hospital,Nishinomiya, Japan.
Otol Neurotol. 2012 Jul;33(5):886-90. doi: 10.1097/MAO.0b013e3182565a27.
To investigate allergic endolymphatic hydrops (EH) and the effect of leukotriene receptor antagonist (LTRA).
Experiment 1: Thirty-six guinea pigs were actively sensitized with DNP-ascaris twice and were provoked with DNP-bovine serum albumin 1 week after the second sensitization. Alterations in the inner ear were investigated histologically at 1, 12, 24, and 36 hours after the provocation, and changes of the endolymphatic space were quantitatively assessed. The animals in the control group received no sensitization but only distilled water. Experiment 2: Twenty-four guinea pigs were actively sensitized and provoked in the same manner. Animals received oral administration of LTRA 1 hour before the provocation. Alterations in the inner ear were investigated as same manner as in Experiment 1. Experiment 3: Eleven of 19 guinea pigs were actively sensitized and provoked in the same manner. Eight animals in the control group received distilled water. One hour after these procedures, the changes in p-AVP levels were investigated.
Experiment 1: EH was observed 12, 24, and 36 hours after the last sensitization. In these groups, their cross-sectional areas of the scala media were significantly larger than that of the control group. Degranulation of mast cells was observed in the endolymphatic sac. Experiment 2: In animal groups with LTRA, EH was not observed at all. Experiment 3: P-AVP levels were significantly elevated in animals with the sensitization.
The sensitization with DNP-Ascaris produced allergic EH and elevation of p-AVP, and allergic EH was inhibited by LTRA.
研究过敏性感音性内淋巴积水（EH）和白三烯受体拮抗剂（LTRA）的作用。
实验 1：36 只豚鼠用 DNP-蛔虫两次主动致敏，第二次致敏后 1 周用 DNP-牛血清白蛋白激发。激发后 1、12、24 和 36 小时，通过组织学观察内耳的变化，并对内淋巴间隙的变化进行定量评估。对照组动物未进行致敏，仅给予蒸馏水。实验 2：24 只豚鼠以相同方式主动致敏和激发。激发前 1 小时，动物接受 LTRA 口服治疗。通过与实验 1 相同的方式研究内耳的变化。实验 3：19 只豚鼠中的 11 只以相同方式主动致敏和激发。对照组 8 只动物给予蒸馏水。激发后 1 小时，研究 p-AVP 水平的变化。
实验 1：EH 在最后一次致敏后 12、24 和 36 小时观察到。在这些组中，中阶的横截面积明显大于对照组。在内淋巴囊中观察到肥大细胞脱颗粒。实验 2：在接受 LTRA 的动物组中，EH 完全没有观察到。实验 3：致敏动物的 p-AVP 水平显著升高。
用 DNP-蛔虫致敏可产生过敏性感音性内淋巴积水和 p-AVP 升高，LTRA 可抑制过敏性感音性内淋巴积水。
