RNA合成过程中叔丁基二甲基硅烷基（TBS）基团的安全脱保护策略。
Safe deprotection strategy for the tert-butyldimethylsilyl (TBS) group during RNA synthesis.
作者信息
Zewge Daniel
机构信息
Department of Process Research, Merck Research Laboratories, Rahway, New Jersey, USA.
出版信息
Curr Protoc Nucleic Acid Chem. 2012 Jun;Chapter 3:Unit3.21. doi: 10.1002/0471142700.nc0321s49.
PMID:22700337
Abstract
This unit describes two protocols for the deprotection of 2'-O-TBS groups in oligoribonucleotides under mild conditions. Desilylation using ammonium fluoride is applicable to fully protected "RNA only" substrates and desilylation using potassium fluoride is applicable to "mixed RNA/non-RNA" substrates. Characterization of products is accomplished using LC/MS, RP HPLC and SAX HPLC.
摘要
本单元描述了在温和条件下对寡核糖核苷酸中2'-O-TBS基团进行脱保护的两种方法。使用氟化铵进行去硅烷基化适用于完全保护的“仅RNA”底物，使用氟化钾进行去硅烷基化适用于“RNA/非RNA混合”底物。产物的表征通过液相色谱/质谱、反相高效液相色谱和强阴离子交换高效液相色谱来完成。
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