Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC, USA.
Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2012 Sep-Oct;4(5):511-24. doi: 10.1002/wnan.1177. Epub 2012 Jun 14.
Viral nanoparticles (VNPs) are attractive platforms for use in the biotechnology and biomedical fields because of their biological nature. A wide variety of these particles, labeled with fluorescent reporters, have been characterized using flow cytometry and cellular imaging techniques. Fluorescence microscopy allows the direct observation of VNPs on the cell surface or inside the membrane as well as the cellular localization of the nanoparticles while flow cytometry allows the statistical quantification of nanoparticle uptake and targeting specificity. These techniques are essential when characterizing the properties of VNPs and provide information toward the use of VNPs for targeting, imaging, and/or cargo delivery.
病毒纳米颗粒（VNPs）因其生物特性而成为生物技术和生物医学领域中极具吸引力的应用平台。这些被荧光报告基团标记的颗粒种类繁多，已通过流式细胞术和细胞成像技术进行了表征。荧光显微镜可以直接观察到细胞表面或膜内的 VNPs，以及纳米颗粒的细胞定位，而流式细胞术则可以对纳米颗粒的摄取和靶向特异性进行统计定量。在表征 VNPs 的性质时，这些技术是必不可少的，并为 VNPs 用于靶向、成像和/或货物输送提供了信息。
