Department of Physiology, Faculty of Biology, University of Murcia, Murcia, Spain.
Physiol Behav. 2014 Jan 17;123:231-5. doi: 10.1016/j.physbeh.2012.06.005. Epub 2012 Jun 15.
The aim of the present work was to investigate associations in circadian markers, melatonin (MT) and cortisol, with metabolic syndrome (MetS) parameters, and with leptin, adiponectin and ghrelin plasma values.
The study was conducted in 70 women (mean age: 41±10years) that were classified without MetS (n=30) and with MetS (n=40). Blood collection, plasma separation and processing, and biochemical analyses for plasma lipids were performed. For measuring salivary melatonin, participants collected two samples. The first simple was obtained before lunch (at 14:00 p.m.) and the second sample was taken at night (3:00 a.m.). On a random working day, participants delivered repeated salivary cortisol samples. The first sample was obtained in the morning (09:00 a.m.), then before lunch at (14:00 p.m.), and finally just before bedtime (23:00 p.m.).
Significant differences were found between the MT measurements taken at night in women without and with MetS. With respect to cortisol, significant differences were found in the different times cortisol levels toward a more flattened pattern among MetS women. Both parameters were positive correlated between them. Of note MT and cortisol night/morning ratios were associated with MetS score and metabolic syndrome components.
The findings indicate that diminished daily amplitude in MT and cortisol circadian patterns was associated with metabolic disturbances in blood pressure, glucose and plasma lipids regulation, ghrelin and adipocyte-secreted hormones such as leptin and adiponectin.
本研究旨在探讨昼夜节律标志物褪黑素（MT）和皮质醇与代谢综合征（MetS）参数的关系，以及与瘦素、脂联素和胃饥饿素血浆值的关系。
该研究在 70 名女性（平均年龄：41±10 岁）中进行，这些女性被分为无 MetS（n=30）和有 MetS（n=40）两组。采集血液样本、分离和处理血浆，并进行血浆脂质生化分析。为了测量唾液褪黑素，参与者采集了两个样本。第一个样本在午餐前（下午 2 点）采集，第二个样本在夜间（凌晨 3 点）采集。在一个随机工作日，参与者多次采集唾液皮质醇样本。第一个样本在早上（上午 9 点）采集，然后在午餐前（下午 2 点）采集，最后在睡前（晚上 11 点）采集。
无 MetS 和有 MetS 的女性夜间 MT 测量值存在显著差异。关于皮质醇，MetS 女性的不同时间点的皮质醇水平存在显著差异，表现出更平坦的模式。这两个参数之间存在正相关。值得注意的是，MT 和皮质醇夜间/清晨比值与 MetS 评分和代谢综合征成分相关。
研究结果表明，MT 和皮质醇昼夜节律模式的日振幅降低与血压、血糖和血浆脂质调节、胃饥饿素和脂肪细胞分泌的激素（如瘦素和脂联素）的代谢紊乱有关。
