Institute of Biotechnology, Faculty of Agriculture, University of Shiraz, Shiraz 71441-65186, Iran.
Food Chem Toxicol. 2012 Sep;50(9):3079-85. doi: 10.1016/j.fct.2012.06.002. Epub 2012 Jun 13.
The inhibitory effects of Zataria multiflora essential oil on nitric oxide (NO) and hydrogen peroxide (H(2)O(2)) production were examined in human monocytes cultured in the presence of 20mM glucose. Z. multiflora essential oil was extracted by water-distillation and then analyzed by GC-MS. Carvacrol (29.2%), thymol (25.4%), p-cymene (11.2%), linalool (9.6%) and γ-terpinene (8%) were the main components detected in the essential oil. Cells cultured in the presence of 20mM glucose showed an increase in NO and H(2)O(2) production as well as NO synthase (NOS) and NADH oxidase (NOX) activities compared to cells cultured in the presence of 5mM glucose. Pretreatment with Z. multiflora essential oil, carvacrol and thymol reduced NO and H(2)O(2) production as well as NOS and NOX activities in those cells cultured in the presence of 20mM glucose. However, p-cymene, linalool and γ-terpinene did not show any such activities. Accordingly, it was concluded that Z. multiflora can reduce oxidative stress and can be used in the therapy of oxidative damage accompanying hyperglycemia and some inflammatory conditions.
采用水蒸馏法提取香薷属植物精油，并用 GC-MS 进行分析。检测到精油的主要成分有：香芹酚（29.2%）、百里酚（25.4%）、对伞花烃（11.2%）、芳樟醇（9.6%）和γ-萜品烯（8%）。与在 5mM 葡萄糖存在下培养的细胞相比，在 20mM 葡萄糖存在下培养的细胞中 NO 和 H2O2 的产生以及一氧化氮合酶（NOS）和烟酰胺腺嘌呤二核苷酸氧化酶（NOX）的活性均增加。用香薷属植物精油、香芹酚和百里酚预处理可降低在 20mM 葡萄糖存在下培养的细胞中 NO 和 H2O2 的产生以及 NOS 和 NOX 的活性。然而，对伞花烃、芳樟醇和γ-萜品烯则没有显示出这种活性。因此，结论是香薷属植物可以减轻氧化应激，可以用于治疗高血糖和一些炎症伴随的氧化损伤。
