Debruyckere G, Van Peteghem C, De Brabander H F, Debackere M
Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, State University of Ghent, Belgium.
Vet Q. 1990 Oct;12(4):246-50. doi: 10.1080/01652176.1990.9694273.
The presence of 17 beta-19-nortestosterone (nandrolone, NT, 17 beta-19-NT) and its epimer 17 alpha-19-nortestosterone (epiNT, 17 alpha-19-NT) was investigated in the urine of six untreated boars, obtained from experimental farms. The presence of 17 beta-19-nortestosterone was screened by RIA and HPTLC and confirmed by GC-MS analysis. Additionally, the two epimers (NT and epiNT) were investigated in the urine of a boar (two-year-old miniature male pig weighing 50 kg) before and after injection of 100 mg Laurabolin (nortestosterone laurate, Intervet N.V., Belgium). The isolation of the steroids was based on sample clean-up with solid phase extraction and subsequent high-performance liquid chromatography. Both gas chromatographic retention data and mass spectrometric data (selected ion monitoring and full spectrum) were used for detection and identification. The presence of 17 beta-19-nortestosterone in the urine of the boars that were not injected proves the endogenous production of the steroid. The absence of the 17 alpha-epimer in the urine of the injected boar suggests that 17 alpha-19-nortestosterone is not a major metabolite of 17 beta-19-nortestosterone.
对从实验农场获取的6头未经处理的公猪尿液中17β - 19 - 去甲睾酮（诺龙，NT，17β - 19 - NT）及其差向异构体17α - 19 - 去甲睾酮（表诺龙，epiNT，17α - 19 - NT）的存在情况进行了研究。通过放射免疫分析（RIA）和高效薄层色谱法（HPTLC）对17β - 19 - 去甲睾酮的存在情况进行筛查，并通过气相色谱 - 质谱联用（GC - MS）分析进行确认。此外，在一头公猪（体重50 kg的两岁小型雄性猪）注射100 mg 劳勃林（月桂酸诺龙，比利时英特威国际有限公司）之前和之后的尿液中，对这两种差向异构体（NT和epiNT）进行了研究。类固醇的分离基于用固相萃取进行样品净化以及随后的高效液相色谱法。气相色谱保留数据和质谱数据（选择离子监测和全谱）均用于检测和鉴定。未注射公猪尿液中17β - 19 - 去甲睾酮的存在证明了该类固醇的内源性产生。注射公猪尿液中不存在17α差向异构体表明17α - 19 - 去甲睾酮不是17β - 19 - 去甲睾酮的主要代谢产物。
