Vandenbroeck M, Van Vyncht G, Gaspar P, Dasnois C, Delahaut P, Pelzer G, De Graeve J, Maghuin-Rogister G
Laboratoire d'Analyse des Denrées Alimentaires d'Origine Animale, Faculté de Médecine Vétérinaire, Université de Liège, Sart Tilman, Belgium.
J Chromatogr. 1991 Apr 5;564(2):405-12. doi: 10.1016/0378-4347(91)80509-b.
To monitor the illegal use of 19-nortestosterone as an anabolizing agent in meat-production, the Belgian Institute of Veterinary Expertise applies a strategy of urine control by radioimmunoassay, positive samples being confirmed by thin-layer chromatography. We have evaluated this control strategy, using gas chromatography-mass spectrometry to confirm the presence of 19-nortestosterone, or its metabolite oestrane-diol, in positive samples from radioimmunoassay. Our results show that the effective way of proceeding remains reliable in cattle, for mature and immature males as well as non-pregnant females, and in pigs, for pregnant and non-pregnant sows. The possible presence of endogenous 19-nortestosterone in cattle, in pregnant cows urine, and in pigs, in boars and in cryptorchid pigs, impedes the control of the use of 19-nortestosterone on these samples. False-positive (not confirmed by gas chromatography-mass spectrometry) results were produced by radioimmunoassay in the urine of castrated pigs and sheep.
为监测19-去甲睾酮作为肉类生产中同化剂的非法使用情况，比利时兽医专业研究所采用了一种通过放射免疫分析法进行尿液检测的策略，阳性样本通过薄层色谱法进行确认。我们使用气相色谱-质谱联用技术对放射免疫分析法检测出的阳性样本中19-去甲睾酮或其代谢产物雌烷二醇的存在情况进行了评估，以此来评价这种检测策略。我们的结果表明，对于牛（包括成年和未成年雄性以及未怀孕雌性）和猪（包括怀孕和未怀孕母猪），现行的有效检测方法仍然可靠。牛的尿液（尤其是怀孕母牛的尿液）、猪的尿液（尤其是公猪和隐睾猪的尿液）中可能存在内源性19-去甲睾酮，这对这些样本中19-去甲睾酮使用情况的检测造成了阻碍。放射免疫分析法在去势猪和绵羊的尿液中产生了假阳性结果（气相色谱-质谱联用技术未确认）。
