National Institute of Crop Science, National Agriculture and Food Research Organization, 2-1-18 Kannondai, Tsukuba 305-8518, Japan.
Amino Acids. 2013 Feb;44(2):345-59. doi: 10.1007/s00726-012-1338-3. Epub 2012 Jun 17.
Drought is a severe environmental constraint to plant productivity and an important factor limiting barley yield. To investigate the initial response of barley to drought stress, changes in protein profile were analyzed using a proteomics technique. Three-day-old barley seedlings of sensitive genotype 004186 and tolerant genotype 004223 were given two treatments, one with 20 % polyethylene glycol and the second with drought induced by withholding water. After 3 days of treatments, proteins were extracted from shoots and separated by 2-dimensional polyacrylamide gel electrophoresis. Metabolism related proteins were decreased in the sensitive genotype under drought; however, they were increased in the tolerant genotype. Photosynthetic related proteins were decreased and increased among the three sensitive and three tolerant genotypes, respectively. In addition, amino acid synthesis and degradation related proteins were increased and decreased among the three tolerant genotypes. These results suggest that chloroplastic metabolism and energy related proteins might play a significant role in the adaptation process of barley seedlings under drought stress.
干旱是植物生产力的严重环境限制因素，也是限制大麦产量的重要因素。为了研究大麦对干旱胁迫的初始响应，使用蛋白质组学技术分析了蛋白质谱的变化。对敏感基因型 004186 和耐受基因型 004223 的 3 天大的大麦幼苗进行了两种处理，一种用 20%聚乙二醇处理，另一种用断水处理诱导干旱。处理 3 天后，从芽中提取蛋白质，并通过二维聚丙烯酰胺凝胶电泳分离。在干旱条件下，敏感基因型中的代谢相关蛋白减少；然而，在耐受基因型中增加了。在三个敏感和三个耐受基因型中，光合作用相关蛋白分别减少和增加。此外，氨基酸合成和降解相关蛋白在三个耐受基因型中增加和减少。这些结果表明，质体代谢和与能量相关的蛋白质可能在大麦幼苗适应干旱胁迫过程中发挥重要作用。
