Department of Pulmonary Diseases, Ordu State Hospital, Ordu, Turkey.
Inflammation. 2012 Oct;35(5):1732-41. doi: 10.1007/s10753-012-9491-0.
Resveratrol has a preventive potential on bleomycin-induced pulmonary fibrosis in prophylactic use; however, it was not studied in the treatment of the fibrosis. This study investigated the role of resveratrol on the treatment of bleomycin-induced pulmonary fibrosis. Intratracheal bleomycin (2.5 mg/kg) was given in fibrosis groups and saline in controls. First dose of resveratrol was given 14 days after bleomycin and continued until sacrifice. On 29th day, fibrosis in lung was estimated by Aschoft's criteria and hydroxyproline content. Bleomycine increased the fibrosis score (3.70 ± 1.04) and hydroxyproline levels (4.99 ± 0.90 mg/g tissue) as compared to control rats (1.02 ± 0.61 and 1.88 ± 0.59 mg/g), respectively. These were reduced to 3.16 ± 1.58 (P = 0.0001) and 3.08 ± 0.73 (P > 0.05), respectively, by resveratrol. Tissue malondialdehyde levels in the bleomycin-treated rats were higher (0.55 ± 0.22 nmol/mg protein) than that of control rats (0.16 ± 0.07; P = 0.0001) and this was reduced to 0.16 ± 0.06 by resveratrol (P = 0.0001). Tissue total antioxidant capacity is reduced (0.027 ± 0.01) by bleomycine administration when compared control rats (0.055 ± 0.012 mmol Trolox Equiv/mg protein; P = 0.0001) and increased to 0.041 ± 0.008 (P = 0.001) by resveratrol. We concluded that resveratrol has some promising potential on the treatment of bleomycin-induced pulmonary fibrosis in rats. However, different doses of the drug should be further studied.
白藜芦醇在预防性使用时有预防博莱霉素诱导的肺纤维化的潜力；然而，它尚未在纤维化的治疗中进行研究。本研究探讨了白藜芦醇在治疗博莱霉素诱导的肺纤维化中的作用。纤维化组给予博莱霉素（2.5mg/kg）气管内给药，对照组给予生理盐水。白藜芦醇的第一剂在博莱霉素后 14 天给予，并持续到处死。第 29 天，通过 Aschoft 标准和羟脯氨酸含量评估肺纤维化。与对照组大鼠（1.02±0.61 和 1.88±0.59mg/g 组织）相比，博莱霉素增加了纤维化评分（3.70±1.04）和羟脯氨酸水平（4.99±0.90mg/g 组织）。通过白藜芦醇，这些分别减少到 3.16±1.58（P=0.0001）和 3.08±0.73（P>0.05）。博莱霉素治疗大鼠的组织丙二醛水平（0.55±0.22nmol/mg 蛋白）高于对照组大鼠（0.16±0.07；P=0.0001），白藜芦醇将其降低至 0.16±0.06（P=0.0001）。与对照组大鼠（0.055±0.012mmol Trolox 等效/mg 蛋白）相比，博莱霉素给药降低了组织总抗氧化能力（0.027±0.01），白藜芦醇将其提高至 0.041±0.008（P=0.001）。我们得出结论，白藜芦醇对大鼠博莱霉素诱导的肺纤维化的治疗具有一定的潜在作用。然而，应该进一步研究该药物的不同剂量。
