The MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, People's Republic of China.
Glycobiology. 2012 Oct;22(10):1343-52. doi: 10.1093/glycob/cws096. Epub 2012 Jun 15.
An agaran-type polysaccharide, GFP08, isolated from Grateloupia filicina (C. Agardh) Lamouroux, was mainly composed of 1,3-linked β-D-galactose partially sulfated at position O-2 and 1,4-linked α-L-galactose O-2, O-3-disulfate, α-L-galactose O-6-sulfate and 3,6-anhydro-α-L-galactose. Small quantities of xylose, 4,6-O-(1'-carboxyethylidene) and 6-O-methyl-β-D-galactose were also present. In mice bearing sarcoma-180 cells, GFP08 decreased tumor weight in a dose-dependent manner. The antiangiogenic activity of GFP08 was evaluated using the chicken chorioallantoic membrane assay, and the results showed that GFP08 dose-dependently reduced new vessel formation. Meanwhile, GFP08 inhibited the differentiation of human umbilical vein endothelial cells (HUVECs) into capillary-like structures in vitro and reduced the number of migrated cells. However, there was no observed cytotoxicity of GFP08 toward HUVECs. Further study revealed that GFP08 decreased tissue factor (TF) expression without affecting the activities of matrix metalloproteinase-2 and -9. All those data indicated that GFP08 had an antitumor effect that might be associated in part with its antiangiogenic effect through down-regulating the expression of TF protein.
从石莼属（C. Agardh）中分离出的琼脂型多糖 GFP08 主要由部分硫酸化的 1,3-连接的β-D-半乳糖和 1,4-连接的α-L-半乳糖 O-2、O-3-二硫酸盐、α-L-半乳糖 O-6-硫酸盐和 3,6-脱水-α-L-半乳糖组成。还存在少量的木糖、4,6-O-(1'-羧乙基)和 6-O-甲基-β-D-半乳糖。在荷肉瘤 180 细胞的小鼠中，GFP08 呈剂量依赖性降低肿瘤重量。使用鸡胚绒毛尿囊膜试验评估 GFP08 的抗血管生成活性，结果表明 GFP08 呈剂量依赖性减少新血管形成。同时，GFP08 抑制体外人脐静脉内皮细胞（HUVEC）分化为毛细血管样结构，并减少迁移细胞的数量。然而，GFP08 对 HUVEC 没有观察到细胞毒性。进一步的研究表明，GFP08 降低了组织因子（TF）的表达，而不影响基质金属蛋白酶-2 和 -9 的活性。所有这些数据表明，GFP08 具有抗肿瘤作用，其可能部分与其抗血管生成作用有关，通过下调 TF 蛋白的表达。
