用于加速液滴中反应的微型磁性陀螺混合器。
Micro Magnetic Gyromixer for Speeding up Reactions in Droplets.
作者信息
Zhang Yi, Wang Tza-Huei
机构信息
Department of Biomedical Engineering, Johns Hopkins University. Clark 122, 3400 North Charles Street, Baltimore, MD 21218, USA.
出版信息
Microfluid Nanofluidics. 2012 Mar 1;12(5):787-794. doi: 10.1007/s10404-011-0922-2. Epub 2011 Dec 6.
We report a novel micro magnetic gyromixer designed for accelerating mixing hence reactions in droplets. The gyromixer is fabricated with magnetite-PDMS composite using soft lithography. The mixer spins and balances itself on the droplet surface through the gyroscopic effect, rapidly homogenizing the enclosed reagents by stretching and folding internal fluid streamlines to enhance mixing. We examined the capability of the gyromixer for improving biochemical reactions in droplets by monitoring the biotin-streptavidin binding as a linker in a quantum dot fluorescence resonant energy transfer (QD-FRET) sensing system. The remotely controlled gyromixer exhibits high flexibility and potential for integration in a variety of droplet-based miniaturized total analysis systems (μTAS) to reduce turnaround times.
我们报道了一种新型的微磁陀螺混合器，其设计目的是加速混合，从而促进液滴中的反应。该陀螺混合器采用磁铁矿-聚二甲基硅氧烷（magnetite-PDMS）复合材料通过软光刻技术制造而成。混合器通过陀螺效应在液滴表面旋转并保持平衡，通过拉伸和折叠内部流体流线来快速均匀化封闭的试剂，从而增强混合效果。我们通过监测生物素-链霉亲和素结合作为量子点荧光共振能量转移（QD-FRET）传感系统中的连接物，研究了陀螺混合器改善液滴中生化反应的能力。这种远程控制的陀螺混合器具有高度的灵活性，并且有潜力集成到各种基于液滴的微型全分析系统（μTAS）中，以减少周转时间。
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