Fitzner A, Niedbalski W, Paprocka G, Kesy A
Department of Foot and Mouth Disease, National Veterinary Research Institute, 98-220 Zduńska Wola, Wodna 7, Poland.
Pol J Vet Sci. 2012;15(1):21-9. doi: 10.2478/v10181-011-0109-5.
In order to determine the genetic variability of Polish RHD virus strains and to confirm the presence of genetic variant (RHDVa) subtype the partial nucleotide sequences of capsid protein gene, including two highly variable regions C and E, were examined. Phylogenetic analyses of 15 viral strains obtained over 18 years revealed the presence of three genetic groups. The oldest RHDV strains exhibit very close amino acid sequence similarity (98-99%) to the German FRG89 reference strain and most of European strains of the same period, as well as Chinese isolate from 1984. The HA-negative strains and isolates with variable reactivity in the HA test belong to the second subgroup and exhibit an intermediate level of variability (about 3%) in the analysed VP60 gene fragment. The most genetically variable strains (6-7%) clustered to RHDVa subtype. The analysis of nucleotides and amino acid sequences demonstrated three pairs of well conserved RHDV strains, isolated over 3, 6 and 10-year period.
为了确定波兰兔出血症病毒（RHDV）毒株的遗传变异性，并确认遗传变异型（RHDVa）亚型的存在，对衣壳蛋白基因的部分核苷酸序列进行了检测，包括两个高变区C和E。对18年间获得的15个病毒毒株进行的系统发育分析显示存在三个遗传组。最古老的RHDV毒株与德国FRG89参考毒株以及同期的大多数欧洲毒株，还有1984年的中国分离株表现出非常接近的氨基酸序列相似性（98-99%）。血凝（HA）阴性毒株以及在HA试验中反应性可变的分离株属于第二个亚组，并且在分析的VP60基因片段中表现出中等水平的变异性（约3%）。遗传变异性最高的毒株（6-7%）聚集到RHDVa亚型。对核苷酸和氨基酸序列的分析表明，有三对RHDV毒株在3年、6年和10年期间分离得到，它们具有良好的保守性。
