Department of Biology & Evolution, University of Ferrara, St. Borsari, Ferrara, Italy.
Parasite Immunol. 2012 Nov;34(11):511-9. doi: 10.1111/j.1365-3024.2012.01373.x.
A histochemical and ultrastructural investigation of the cellular inflammatory response within the intestines of tench Tinca tinca L. naturally infected with the caryophyllidean cestode Monobothrium wageneri was conducted and the data obtained compared to those in uninfected counterparts. Cestode infections within the intestines were evident through the appearance of raised inflammatory swellings induced by the deep penetration of their scolices into the intestinal wall. Cestodes typically attached in tight clusters, inducing a massive hyperplastic granulocyte response of mast cells and neutrophils, which were significantly more numerous (P < 0·01) in the intestines of infected (n = 14) than of uninfected (n = 9) tench. Neutrophils were more abundant than mast cells (P < 0·01) in host tissues in close proximity to the parasite tegument. In transmission electron microscopy sections, mast cells and neutrophils were frequently observed in contact with or inside capillaries, and in close proximity to the cestode. Degranulation of both cell types was seen in the submucosa and lamina muscularis, notably in the immediate tissues surrounding the scolex of M. wageneri. No tegumental secretions were seen at the host-parasite interface. Occasional rodlet cells were encountered in the submucosa of infected fish.
对自然感染 Caryophyllidea 绦虫 Monobothrium wageneri 的欧鳗肠道内细胞炎症反应进行了组织化学和超微结构研究，并将获得的数据与未感染的对照组进行了比较。通过绦虫头节深深穿透肠壁引起的凸起性炎症肿胀，可以明显看出肠道内的绦虫感染。绦虫通常紧密聚集，引起大量肥大细胞和嗜中性粒细胞的过度增生反应，在感染（n=14）的欧鳗肠道中，这些细胞的数量明显多于未感染（n=9）的欧鳗（P<0·01）。在靠近寄生虫体被膜的宿主组织中，嗜中性粒细胞比肥大细胞更丰富（P<0·01）。在透射电子显微镜切片中，经常观察到肥大细胞和嗜中性粒细胞与毛细血管接触或位于其中，并与绦虫密切相关。在黏膜下层和肌层中观察到这两种细胞类型的脱颗粒现象，在 M. wageneri 的头节周围的组织中尤为明显。在宿主-寄生虫界面未观察到体被分泌产物。在感染鱼的黏膜下层偶尔会遇到杆状细胞。
