Dezfuli B S, Bo T, Lorenzoni M, Shinn A P, Giari L
Department of Life Sciences and Biotechnology, University of Ferrara, Italy.
Department of Science and Technological Innovation, University of Piemonte Orientale, Italy.
Vet Parasitol. 2015 Mar 15;208(3-4):272-9. doi: 10.1016/j.vetpar.2015.01.002. Epub 2015 Jan 14.
A series of ultrastructural-based studies were conducted on the interface region in different fish-helminth systems: (a) an intestinal infection of the cestode Monobothrium wageneri in tench, Tinca tinca; (b) an extensive intestinal submucosa and mucosal infection in tench by metacercariae of an unidentified digenean trematode; (c) an intestinal infection in brown trout, Salmo trutta, by the acanthocephalan Dentitruncus truttae; (d) an extraintestinal infection by larvae of the acanthocephalan, Pomphorhynchus laevis in three-spined sticklebacks, Gasterosteus aculeatus; and (e) an infection in the livers of Eurasian minnow, Phoxinus phoxinus, by larvae of the nematode Raphidascaris acus. Endoparasitic helminths frequently cause inflammation of the digestive tract and associated organs, inducing the recruitment of various immune cells to the site of infection. In each of the fish-helminth systems that were studied, a massive hyperplastic granulocyte response involving mast cells (MCs) and neutrophils in close proximity to the helminths was documented. The current study presents data on the interface region in each fish-helminth system and documents the penetration of mast cells granules within the tegument of P. laevis larvae. No extracellular vesicles containing tegumental secretions from any of the four different taxa of endoparasitic helminths species at the host-parasite interface region were seen.
对不同鱼类-蠕虫系统的界面区域进行了一系列基于超微结构的研究：(a) 丁鱥（Tinca tinca）感染绦虫瓦氏单叶绦虫（Monobothrium wageneri）的肠道感染；(b) 丁鱥被一种未鉴定的复殖吸虫尾蚴广泛感染肠道黏膜下层和黏膜；(c) 褐鳟（Salmo trutta）感染棘头虫特鲁塔齿棘头虫（Dentitruncus truttae）的肠道感染；(d) 三刺鱼（Gasterosteus aculeatus）感染棘头虫平滑膨吻棘头虫（Pomphorhynchus laevis）幼虫的肠外感染；以及(e) 欧鳑鲏（Phoxinus phoxinus）肝脏被线虫尖锐似蛔线虫（Raphidascaris acus）幼虫感染。体内寄生虫经常引起消化道及相关器官的炎症，促使各种免疫细胞募集到感染部位。在所研究的每个鱼类-蠕虫系统中，均记录到在蠕虫附近有肥大细胞（MCs）和中性粒细胞参与的大量增生性粒细胞反应。本研究展示了每个鱼类-蠕虫系统界面区域的数据，并记录了肥大细胞颗粒穿透平滑膨吻棘头虫幼虫的皮层。在宿主-寄生虫界面区域，未观察到来自四种不同类群的体内寄生虫的含有皮层分泌物的细胞外囊泡。
