Technical University of Lodz, Institute of Applied Radiation Chemistry, Laboratory of Laser Molecular Spectroscopy, Wroblewskiego 15, 93-590 Lodz, Poland.
Technol Cancer Res Treat. 2012 Aug;11(4):317-31. doi: 10.7785/tcrt.2012.500280. Epub 2012 Jun 15.
There is a considerable interest in the developing new diagnostic techniques allowing noninvasive tracking of the progress of therapies used to treat a cancer. Raman imaging of distribution of phthalocyanine photosensitizers may open new possibilities of Photodynamic Therapy (PDT) to treat a wide range of neoplastic lesions with improved effectiveness of treatment through precise identification of malignant areas. We have employed Raman imaging and Raman spectroscopy to analyze human breast cancer tissue that interacts with photosensitizers used in the photodynamic therapy of cancer. PCA (Principal Component Analysis) has been employed to analyze various areas of the noncancerous and cancerous breast tissues. The results show that the emission spectra combined with the Raman images are very sensitive indicators to specify the aggregation state and the distribution of phthalocyanines in the cancerous and noncancerous breast tissues. Our results provide experimental evidence on the role of aggregation of phthalocyanines as a factor of particular significance in differentiation of the normal and tumourous (cancerous or benign pathology) breast tissues. We conclude that the Raman imaging reported here has a potential to be a novel and effective photodynamic therapeutic method with improved selectivity for the treatment of breast cancer.
人们对开发新的诊断技术很感兴趣，这些技术可以无创地跟踪用于治疗癌症的疗法的进展。酞菁类光动力治疗剂的拉曼成像可能为光动力治疗（PDT）开辟新的可能性，通过精确识别恶性区域，提高治疗效果，从而治疗广泛的肿瘤病变。我们已经使用拉曼成像和拉曼光谱分析了与人乳腺癌组织相互作用的光动力治疗癌症的光敏剂。主成分分析（PCA）已被用于分析非癌性和癌性乳腺组织的各个区域。结果表明，发射光谱与拉曼图像的结合是指定癌性和非癌性乳腺组织中酞菁聚集状态和分布的非常敏感的指标。我们的结果提供了实验证据，证明了酞菁的聚集作为区分正常和肿瘤（癌性或良性病理）乳腺组织的重要因素的作用。我们得出结论，这里报道的拉曼成像是一种有潜力的新型有效的光动力治疗方法，对治疗乳腺癌具有更高的选择性。
