Abramczyk Halina, Brozek-Pluska Beata, Surmacki Jakub, Musial Jacek, Kordek Radzislaw
Lodz University of Technology, Institute of Applied Radiation Chemistry, Laboratory of Laser Molecular Spectroscopy, Wroblewskiego 15, 93-590 Lodz, Poland.
Analyst. 2014 Nov 7;139(21):5547-59. doi: 10.1039/c4an00966e.
Raman microspectroscopy and confocal Raman imaging combined with confocal fluorescence were used to study the distribution and aggregation of aluminum tetrasulfonated phthalocyanine (AlPcS4) in noncancerous and cancerous breast tissues. The results demonstrate the ability of Raman spectroscopy to distinguish between noncancerous and cancerous human breast tissue and to identify differences in the distribution and aggregation of aluminum phthalocyanine, which is a potential photosensitizer in photodynamic therapy (PDT), photodynamic diagnosis (PDD) and photoimmunotherapy (PIT) of cancer. We have observed that the distribution of aluminum tetrasulfonated phthalocyanine confined in cancerous tissue is markedly different from that in noncancerous tissue. We have concluded that Raman imaging can be treated as a new and powerful technique useful in cancer photodynamic therapy, increasing our understanding of the mechanisms and efficiency of photosensitizers by better monitoring localization in cancer cells as well as the clinical assessment of the therapeutic effects of PDT and PIT.
采用拉曼光谱显微镜和共聚焦拉曼成像结合共聚焦荧光技术，研究了四磺化铝酞菁（AlPcS4）在非癌性和癌性乳腺组织中的分布与聚集情况。结果表明，拉曼光谱能够区分人非癌性和癌性乳腺组织，并识别酞菁铝（一种在癌症光动力疗法（PDT）、光动力诊断（PDD）和光免疫疗法（PIT）中具有潜在应用价值的光敏剂）在分布和聚集上的差异。我们观察到，局限于癌性组织中的四磺化铝酞菁的分布与非癌性组织中的明显不同。我们得出结论，拉曼成像可被视为一种用于癌症光动力疗法的新型且强大的技术，通过更好地监测癌细胞中的定位以及对PDT和PIT治疗效果的临床评估，增强我们对光敏剂作用机制和效率的理解。
