[NAD+ synthesis in the isolated kidney cell nuclei of dogs after the intravenous administration of amphotericin B].

A study was made of the synthesis of nicotinamide adenine dinucleotide (NAD+) in the nuclei of kidney cells of dogs under normal conditions and upon the effect of the polyenic antibiotic amphotericin B. An active NAD-pyrophosphorylase has been found in the nuclei of kidney cells. It has been established that a intervenous introduction of amphotericin B stimulates NAD+ production. Amphotericin B also causes a decrease in the amount of histones in the nucleus. In the case of the nuclear membrane damage by a non-ionic detergent Triton X-100, no increase in the synthesis of NAD+ has been observed in the nuclei of kidney cells of animals treated with antibiotics, as opposed to the control ones. Under discussion is a question of a possible mechanism of the effect of polyenic antibiotics on the synthesis and metabolic activity of NAD+.