一种适用于植物遗传修饰的 BioBrick 兼容策略。
A BioBrick compatible strategy for genetic modification of plants.
机构信息
Department of Systems Biology, Harvard Medical School, Boston, MA, 02115, USA.
Current Address: Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, CA, 90095, USA.
出版信息
J Biol Eng. 2012 Jun 20;6(1):8. doi: 10.1186/1754-1611-6-8.
BACKGROUND
Plant biotechnology can be leveraged to produce food, fuel, medicine, and materials. Standardized methods advocated by the synthetic biology community can accelerate the plant design cycle, ultimately making plant engineering more widely accessible to bioengineers who can contribute diverse creative input to the design process.
RESULTS
This paper presents work done largely by undergraduate students participating in the 2010 International Genetically Engineered Machines (iGEM) competition. Described here is a framework for engineering the model plant Arabidopsis thaliana with standardized, BioBrick compatible vectors and parts available through the Registry of Standard Biological Parts (http://www.partsregistry.org). This system was used to engineer a proof-of-concept plant that exogenously expresses the taste-inverting protein miraculin.
CONCLUSIONS
Our work is intended to encourage future iGEM teams and other synthetic biologists to use plants as a genetic chassis. Our workflow simplifies the use of standardized parts in plant systems, allowing the construction and expression of heterologous genes in plants within the timeframe allotted for typical iGEM projects.
背景
植物生物技术可以用于生产食品、燃料、药物和材料。合成生物学界所倡导的标准化方法可以加速植物设计周期，最终使更多的生物工程师能够广泛参与到植物工程设计中，为设计过程贡献多样化的创意。
结果
本文主要介绍了在 2010 年国际遗传工程机器设计竞赛（iGEM）中，由本科生参与完成的工作。文中描述了一个使用标准化的、与 BioBrick 兼容的载体和通过标准生物部件注册（http://www.partsregistry.org）可获得的部件来工程改造模式植物拟南芥的框架。该系统被用于构建一个概念验证植物，该植物可以外源性表达味觉反转蛋白奇迹素。
结论
我们的工作旨在鼓励未来的 iGEM 团队和其他合成生物学家将植物作为遗传底盘。我们的工作流程简化了标准化部件在植物系统中的使用，使得在典型的 iGEM 项目规定的时间内，能够在植物中构建和表达异源基因。
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