Di Febo Tiziana, Luciani Mirella, Portanti Ottavio, Bonfini Barbara, Lelli Rossella, Tittarelli Manuela
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise 'G. Caporale', Campo Boario, 64100 Teramo, Italy.
Vet Ital. 2012 Apr-Jun;48(2):133-56.
A competitive enzyme-linked immunosorbent assay (c-ELISA), an indirect ELISA (i-ELISA) and a dissociation-enhanced lanthanide fluorescence immunoassay (DELFIA) were developed to test for antibodies to Brucella suis in pig and wild boar sera. An anti-Brucella-LPS monoclonal antibody (MAb 4B5A) (c-ELISA and DELFIA) and an anti-swine IgG monoclonal antibody (MAb 10C2G5) (i-ELISA) were used for the three assays. The specificity (Sp) and sensitivity (Se) of the assays gave the following results: Se and Sp=100% at a cut-off value of 61.0% (B/B0%) for c-ELISA; Sp=99.1% and Se=100% at a cut-off value of 21.7% (percentage positivity: PP%) for i-ELISA; Sp=91.0% and Se=75% at a cut-off value of 37.0% (B/B0%) for DELFIA. In addition, the performance of a commercial fluorescence polarisation assay (FPA), standardised for bovine sera, was evaluated in swine sera. The specificity and sensitivity obtained were both 100% at a cut-off value of 99.5 (millipolarisation unit values). These results suggest that the combination of c-ELISA, i-ELISA and FPA can be used to improve the serological diagnosis of swine brucellosis.
开发了一种竞争性酶联免疫吸附测定法（c-ELISA）、一种间接酶联免疫吸附测定法（i-ELISA）和一种解离增强镧系元素荧光免疫测定法（DELFIA），用于检测猪和野猪血清中的猪布鲁氏菌抗体。三种测定法分别使用了抗布鲁氏菌脂多糖单克隆抗体（单克隆抗体4B5A）（c-ELISA和DELFIA）和抗猪IgG单克隆抗体（单克隆抗体10C2G5）（i-ELISA）。这些测定法的特异性（Sp）和敏感性（Se）结果如下：c-ELISA在临界值为61.0%（B/B0%）时，Se和Sp均为100%；i-ELISA在临界值为21.7%（阳性百分比：PP%）时，Sp = 99.1%，Se = 100%；DELFIA在临界值为37.0%（B/B0%）时，Sp = 91.0%，Se = 75%。此外，还评估了一种针对牛血清标准化的商业荧光偏振测定法（FPA）在猪血清中的性能。在临界值为99.5（毫偏振单位值）时，获得的特异性和敏感性均为100%。这些结果表明，c-ELISA、i-ELISA和FPA相结合可用于改善猪布鲁氏菌病的血清学诊断。
