埃及开罗省和吉萨省猪体内 spp. 的血清学和分子鉴定
Serological and Molecular Identification of spp. in Pigs from Cairo and Giza Governorates, Egypt.
作者信息
Khan Aman Ullah, Melzer Falk, El-Soally Sherif Abdel Ghafar Elsayed, Elschner Mandy C, Mohamed Shereen Aziz, Sayed Ahmed Mohamed Abdelmonem, Roesler Uwe, Neubauer Heinrich, El-Adawy Hosny
机构信息
Friedrich-Loeffler-Institut, Institute of Bacterial Infections and Zoonoses, 07743 Jena, Germany.
Institute for Animal Hygiene and Environmental Health, Free University of Berlin, 14163 Berlin, Germany.
出版信息
Pathogens. 2019 Nov 20;8(4):248. doi: 10.3390/pathogens8040248.
Brucellosis is considered as endemic disease of animals and humans since thousands of years in Egypt. However, brucellosis in pigs has never been reported in Egypt. Thus, serological and molecular assays were applied to detect anti- antibodies and DNA in serum samples collected from pigs. In total 331 blood samples collected from male and female pigs at slaughterhouses of Cairo and Giza governorates were investigated using c- and i-ELISA and real-time PCR. Anti- antibodies were detected in 16 (4.83%) and 36 (10.8%) sera by i-ELISA and c-ELISA, respectively. DNA was detected in 10 (3.02%) seropositive samples and identified as (7/10) (3/10). A higher prevelance was found in boars. This is the first study investigating pig brucellosis in Egypt. The results of this study will raise awareness for brucellosis in these farm animals and will help to develop effective control strategies.
几千年来，布鲁氏菌病在埃及一直被视为动物和人类的地方病。然而，埃及从未报告过猪的布鲁氏菌病。因此，应用血清学和分子检测方法来检测从猪采集的血清样本中的抗体和DNA。使用c-ELISA和i-ELISA以及实时PCR对从开罗和吉萨省屠宰场的雄性和雌性猪采集的总共331份血液样本进行了调查。通过i-ELISA和c-ELISA分别在16份（4.83%）和36份（10.8%）血清中检测到抗体。在10份（3.02%）血清阳性样本中检测到DNA，并鉴定为（7/10）（3/10）。公猪中的患病率更高。这是埃及第一项调查猪布鲁氏菌病的研究。这项研究的结果将提高对这些农场动物布鲁氏菌病的认识，并有助于制定有效的控制策略。
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