James Madison University, Harrisonburg, VA 22807, United States.
Vet Microbiol. 2012 Nov 9;160(1-2):189-96. doi: 10.1016/j.vetmic.2012.05.026. Epub 2012 Jun 2.
Bordetella avium continues to be an economic issue in the turkey industry as the causative agent of bordetellosis, which often leads to serious secondary infections. This study presents a broad characterization of the antibiotic resistance patterns in this diverse collection of B. avium strains collected over the past thirty years. In addition, the plasmid basis for the antibiotic resistance was characterized. The antibiotic resistance pattern allowed the development of a novel enrichment culture method that was subsequently employed to gather new isolates from diseased turkeys and a healthy sawhet owl. While a healthy turkey flock was shown to seroconvert by four weeks-of-age, attempts to culture B. avium from healthy turkey poults were unsuccessful. Western blot of B. avium strains using pooled serum from diseased and healthy commercial turkey flocks revealed both antigenic similarities and differences between strains. In sum, the work documents the continued exposure of commercial turkey flocks to B. avium and the need for development of an effective, inexpensive vaccine to control spread of the disease.
鸟分枝杆菌仍然是火鸡产业的一个经济问题，因为它是导致鸟分枝杆菌病的病原体，该病常导致严重的继发感染。本研究广泛描述了过去三十年来收集的这种多样化的鸟分枝杆菌菌株的抗生素耐药模式。此外，还对耐药性的质粒基础进行了表征。抗生素耐药模式允许开发一种新的富集培养方法，随后该方法被用于从患病火鸡和健康的锯嘴猫头鹰中收集新的分离株。虽然健康的火鸡群在 4 周龄时就表现出血清转化，但从健康火鸡雏鸡中培养鸟分枝杆菌的尝试均未成功。使用来自患病和健康商业火鸡群的混合血清对鸟分枝杆菌菌株进行 Western blot 分析，揭示了菌株之间存在抗原相似性和差异。总之，这项工作记录了商业火鸡群持续接触鸟分枝杆菌的情况，需要开发一种有效、廉价的疫苗来控制疾病的传播。
