International Center for Materials Nanoarchitectonics-WPI-MANA, National Institute for Materials Science, 1-2-1, Sengen, Tsukuba, Ibaraki 305-0047, Japan.
Int J Biol Macromol. 2012 Nov;51(4):627-31. doi: 10.1016/j.ijbiomac.2012.06.014. Epub 2012 Jun 18.
Electrospinning is a versatile method to fabricate nanofibers of a range of polymeric and composite materials suitable as scaffolds for tissue engineering applications. In this study, we report the fabrication and characterization of polyaniline-carbon nanotube/poly(N-isopropyl acrylamide-co-methacrylic acid) (PANI-CNT/PNIPAm-co-MAA) composite nanofibers and PNIPAm-co-MAA nanofibers suitable as a three-dimensional (3D) conducting smart tissue scaffold using electrospinning. The chemical structure of the resulting nanofibers was characterized with FTIR and ¹H NMR spectroscopy. The surface morphology and average diameter of the nanofibers were observed by SEM. Cellular response of the nanofibers was studied with mice L929 fibroblasts. Cell viability was checked on 7 th day of cell culture by double staining the cells with calcein-AM and PI dye. PANI-CNT/PNIPAm-co-MAA composite nanofibers were shown the highest cell growth and cell viability as compared to PNIPAm-co-MAA nanofibers. Cell viability in the composite nanofibers was obtained in order of 98% that indicates the composite nanofibers provide a better environment as a 3D scaffold for the cell proliferation and attachment suitable for tissue engineering applications.
静电纺丝是一种通用的方法，可以制备各种聚合物和复合材料的纳米纤维，适合用作组织工程应用的支架。在这项研究中，我们报告了聚（苯胺-碳纳米管）/聚（N-异丙基丙烯酰胺-co-甲基丙烯酸）（PANI-CNT/PNIPAm-co-MAA）复合纳米纤维和 PNIPAm-co-MAA 纳米纤维的制备和表征，这些纳米纤维适合用作三维（3D）导电智能组织支架，使用静电纺丝技术。通过傅里叶变换红外光谱（FTIR）和 ¹H 核磁共振光谱（¹H NMR）对所得纳米纤维的化学结构进行了表征。通过扫描电子显微镜（SEM）观察纳米纤维的表面形貌和平均直径。用小鼠 L929 成纤维细胞研究了纳米纤维的细胞反应。通过用 calcein-AM 和 PI 染料双重染色细胞，在细胞培养的第 7 天检查细胞活力。与 PNIPAm-co-MAA 纳米纤维相比，PANI-CNT/PNIPAm-co-MAA 复合纳米纤维显示出最高的细胞生长和细胞活力。复合纳米纤维的细胞活力达到 98%，表明复合纳米纤维为细胞增殖和附着提供了更好的环境，适合组织工程应用。
