College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, People's Republic of China.
Carbohydr Polym. 2013 Jan 2;91(1):419-27. doi: 10.1016/j.carbpol.2012.08.069. Epub 2012 Aug 25.
We report the fabrication of a novel carbon nanotube-containing nanofibrous polysaccharide scaffolding material via the combination of electrospinning and layer-by-layer (LbL) self-assembly techniques for tissue engineering applications. In this approach, electrospun cellulose acetate (CA) nanofibers were assembled with positively charged chitosan (CS) and negatively charged multiwalled carbon nanotubes (MWCNTs) or sodium alginate (ALG) via a LbL technique. We show that the 3-dimensional fibrous structures of the CA nanofibers do not appreciably change after the multilayered assembly process except that the surface of the fibers became much rougher than that before assembly. The incorporation of MWCNTs in the multilayered CA fibrous scaffolds tends to endow the fibers with improved mechanical property and promote fibroblast attachment, spreading, and proliferation when compared with CS/ALG multilayer-assembled fibrous scaffolds. The approach to engineering the nanofiber surfaces via LbL assembly likely provides many opportunities for new scaffolding materials design in various tissue engineering applications.
我们通过静电纺丝和层层自组装技术的结合，报告了一种新型的含碳纳米管的纳米纤维多糖支架材料的制备，用于组织工程应用。在这种方法中，通过层层自组装技术将静电纺丝的醋酸纤维素（CA）纳米纤维与带正电荷的壳聚糖（CS）和带负电荷的多壁碳纳米管（MWCNTs）或海藻酸钠（ALG）组装在一起。我们表明，除了纤维表面变得比组装前粗糙得多之外，多层组装过程对 CA 纳米纤维的 3 维纤维结构没有明显的改变。与 CS/ALG 多层组装纤维支架相比，MWCNTs 的加入使多层 CA 纤维支架具有更好的机械性能，并促进成纤维细胞的附着、伸展和增殖。通过层层自组装对纳米纤维表面进行工程设计的方法可能为各种组织工程应用中新型支架材料的设计提供许多机会。
