Department of Wildlife and Fisheries Sciences and Intercollegiate, Faculty of Nutrition, Texas A&M University, 216 Heep Laboratory Building, 2258 TAMUS, College Station, TX 77843, USA.
Fish Shellfish Immunol. 2012 Sep;33(3):543-51. doi: 10.1016/j.fsi.2012.06.005. Epub 2012 Jun 21.
Channel catfish was used to investigate the enhancement of vaccine efficacy following dietary supplementation with arginine (ARG, 4% of diet), glutamine (GLN, 2% of diet), or a combination of both. After vaccination against Edwardsiella ictaluri, humoral and cellular immune responses, along with lymphoid organ responses were evaluated. E. ictaluri-specific antibody titers in plasma were higher (P < 0.05) in fish fed the supplemented diets compared to those fed the basal diet as early as 7 d post-vaccination (dpv). B-cell proportion in head-kidney was higher (P < 0.05) at 14 dpv in vaccinated fish fed the GLN diet. The responsiveness of spleen and head-kidney lymphocytes against E. ictaluri was enhanced (P < 0.05) by dietary supplementation of ARG or GLN at 14 dpv. Additionally, at 7 dpv, vaccinated fish fed the GLN diet had higher (P < 0.05) head kidney weights relative to the other dietary treatments, and vaccinated fish fed ARG-supplemented diets had higher (P < 0.05) protein content in this tissue. Results from this study suggest that dietary supplementation of ARG and GLN may improve specific cellular and humoral mechanisms, enhancing the acquired immunity in vaccinated channel catfish.
我们用斑点叉尾鮰研究了日粮补充精氨酸（ARG，日粮的 4%）、谷氨酰胺（GLN，日粮的 2%）或两者组合对疫苗效力的增强作用。在对鮰爱德华氏菌进行疫苗接种后，评估了体液和细胞免疫反应以及淋巴器官反应。与基础日粮相比，早在疫苗接种后 7 天（dpv），补充日粮组鱼类的血浆中针对 E. ictaluri 的特异性抗体滴度更高（P < 0.05）。在 GLN 日粮组中，头肾中的 B 细胞比例在 14 dpv 时更高（P < 0.05）。在 14 dpv 时，日粮补充 ARG 或 GLN 增强了脾和头肾淋巴细胞对 E. ictaluri 的反应性（P < 0.05）。此外，在 7 dpv 时，GLN 日粮组的疫苗接种鱼的头肾重量更高（P < 0.05），而补充 ARG 的日粮组的疫苗接种鱼的该组织的蛋白含量更高（P < 0.05）。本研究结果表明，日粮补充 ARG 和 GLN 可能改善了特定的细胞和体液机制，增强了接种斑点叉尾鮰的获得性免疫。
