Kordon Adef O, Abdelhamed Hossam, Karsi Attila, Pinchuk Lesya M
Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS, USA.
Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS, USA.
Dev Comp Immunol. 2021 Mar;116:103950. doi: 10.1016/j.dci.2020.103950. Epub 2020 Nov 28.
We extend the previous findings on the differential activity of immune-related genes in the lymphoid organs of channel catfish in the 7 days post-challenge (dpc) with E. ictaluri live attenuated vaccines (LAVs) and wild type (WT) strains by assessing the expression of these genes in the 21 dpc. The expression of T and B cell-specific genes were significantly elevated in the spleen at 14 dpc and in the AK at 21 dpc in catfish treated with E. ictaluri WT and LAV strains compared to a non-treated control group. The gene expression of IFN-γ correlated with adaptive immunity genes in the lymphoid tissues of catfish. These data indicate that two novel LAVs were able to trigger the activation of T helper1 polarization cytokine IFN-γ gene and specific lymphocyte genes in the spleen followed by their activation in the AK of catfish without causing inflammation, thus providing protective immunity in E. ictaluri infection.
我们通过评估这些基因在攻毒后21天（dpc）的表达，扩展了之前关于斑点叉尾鮰在经迟缓爱德华氏菌活减毒疫苗（LAVs）和野生型（WT）菌株攻毒后7天（dpc）时免疫相关基因在淋巴器官中差异活性的研究结果。与未处理的对照组相比，在用迟缓爱德华氏菌WT和LAV菌株处理的斑点叉尾鮰中，T和B细胞特异性基因的表达在14 dpc时在脾脏中显著升高，在21 dpc时在头肾（AK）中显著升高。干扰素-γ（IFN-γ）的基因表达与斑点叉尾鮰淋巴组织中的适应性免疫基因相关。这些数据表明，两种新型LAVs能够触发斑点叉尾鮰脾脏中辅助性T细胞1极化细胞因子IFN-γ基因和特异性淋巴细胞基因的激活，随后在头肾中激活，且不会引起炎症，从而在迟缓爱德华氏菌感染中提供保护性免疫。
