Department of Pharmacology, School of Pharmacy, Jilin University, Changchun 130021, China.
Int J Biol Macromol. 2012 Nov;51(4):550-4. doi: 10.1016/j.ijbiomac.2012.06.021. Epub 2012 Jun 21.
In this paper, the composition and biological activities of polysaccharides from Inula britannica flower IBP obtained by water extraction were investigated. The properties and chemical compositions of IBP were analyzed with HPLC and IR methods. The results showed that IBP consisted of two kinds of polysaccharides with the molecular weight of 3500 Da, 700 Da. IBP consisted of mannose, glucuronic acid, rhamnose, galacturonic acid, glucose, galactose, arabinose with a molar ratio of 4.1:1:1.4:2.7:14.6:6.3:7.9. The IR spectrum of IBP revealed the typical characteristics of polysaccharides and protein. IBP was administered orally at three doses [100, 200 and 400 mg/kg body weight] for 14 days to the diabetic mice induced by alloxan. The body weight, plasma glucose, serum triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C) and liver glycogen were evaluated in normal and alloxan-induced diabetic mice. IBP could dose-dependently significantly increase the body weight of diabetic mice, and reverse the decrease of plasma glucose, glycogen and the decrease of blood lipid of diabetic mice as compared to those in control group. These results indicated that IBP could be developed to a potential anti-diabetic drug in the future.
本文研究了水提白头翁花 IBP 的组成和生物活性。采用 HPLC 和 IR 方法分析了 IBP 的性质和化学成分。结果表明，IBP 由两种多糖组成，分子量分别为 3500 Da 和 700 Da。IBP 由甘露糖、葡萄糖醛酸、鼠李糖、半乳糖醛酸、葡萄糖、半乳糖、阿拉伯糖组成，摩尔比为 4.1:1:1.4:2.7:14.6:6.3:7.9。IR 光谱显示 IBP 具有多糖和蛋白质的典型特征。IBP 以 100、200 和 400 mg/kg 体重的剂量连续灌胃给药 14 天，对由链脲佐菌素诱导的糖尿病小鼠进行治疗。评估了正常和链脲佐菌素诱导的糖尿病小鼠的体重、血浆葡萄糖、血清甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）和肝糖原。与对照组相比，IBP 可剂量依赖性地显著增加糖尿病小鼠的体重，并逆转糖尿病小鼠血浆葡萄糖、肝糖原和血脂的降低。这些结果表明，IBP 未来可能开发成为一种潜在的抗糖尿病药物。
