Xu Chunping, Yang Chenchen, Mao Duobin
Department of Tobacco Engineering, College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan, P.R. China.
Pharmacogn Mag. 2014 Jan;10(37):66-9. doi: 10.4103/0973-1296.126664.
The metabolic compounds from tobacco were investigated to posses various biological activities, such as antioxidant, antimicrobial and neuroprotective activities.
Polysaccharides were isolated from the ultrasound-assisted extraction of flue-cured tobacco with hot water. The purified polysaccharides were analyzed by GC-MS, FT-IR, and TGA (Thermogravimetric analysis). The antioxidant activities of purified polysaccharides were evaluated in vitro.
Two polysaccharides (Fr-I and Fr-II) were isolated and purified. The analysis of monosaccharide composition in the polysaccharide by GC/MS revealed that Fr-I and Fr-II were heteropolysaccharides. Fr-I consisted of D-mannose and galactose, ribose and arabinose, while Fr-II was mainly composed of glucose, allose, galactose and mannose. Thermo gravimetric analysis (TGA) indicated that the degradation temperature (Td) of the Fr-I (241 °C) was higher than that of Fr-II (220 °C). Both samples showed inhibitory effects on OH (hydroxyl) and DPPH (2, 2-diphenyl-1-picryl-hydrazyl-hydrate) radical in a concentration-dependent manner. Comparing Fr-I with Fr-II, the latter has a strong scavenging ability.
Both polysaccharide fractions showed significant antioxidant effects. Various factors influenced the antioxidant activity of polysaccharides.
烟草中的代谢化合物被研究具有多种生物活性，如抗氧化、抗菌和神经保护活性。
采用热水超声辅助提取法从烤烟中分离多糖。对纯化后的多糖进行气相色谱-质谱联用（GC-MS）、傅里叶变换红外光谱（FT-IR）和热重分析（TGA）。体外评价纯化多糖的抗氧化活性。
分离并纯化出两种多糖（Fr-I和Fr-II）。通过GC/MS分析多糖中的单糖组成表明，Fr-I和Fr-II为杂多糖。Fr-I由D-甘露糖、半乳糖、核糖和阿拉伯糖组成，而Fr-II主要由葡萄糖、阿洛糖、半乳糖和甘露糖组成。热重分析（TGA）表明，Fr-I的降解温度（Td）（241℃）高于Fr-II（220℃）。两个样品均对羟基（OH）和2,2-二苯基-1-苦基肼自由基（DPPH）具有浓度依赖性抑制作用。比较Fr-I和Fr-II，后者具有较强的清除能力。
两种多糖组分均表现出显著的抗氧化作用。多种因素影响多糖的抗氧化活性。
