Departament de Fisiologia, Facultat de Farmàcia, Universitat de Barcelona, Spain.
Pharmacol Res. 2012 Sep;66(3):207-12. doi: 10.1016/j.phrs.2012.05.009. Epub 2012 Jun 21.
Cocoa contains flavonoids with antioxidant properties. The aim of this study was to ascertain the effect of cocoa intake on oxidative stress associated with a model of chronic inflammation such as adjuvant arthritis. Female Wistar rats were fed with a 5% or 10% cocoa-enriched diet or were given p.o. a quercetin suspension every other day for 10 days. Arthritis was induced by a heat-killed Mycobacterium butyricum suspension. Reactive oxygen species (ROS) produced by macrophages, and splenic superoxide dismutase (total, cytoplasmic and mitochondrial) and catalase activities were determined. Clinically, joint swelling in arthritic rats was not reduced by antioxidants; however, the 5% cocoa diet and quercetin administration reduced ROS production. Moreover, the 5% cocoa diet normalized the activities of superoxide dismutase and catalase. In conclusion, a cocoa diet reduces the oxidative stress associated with a chronic inflammatory pathology, although it was not enough to attenuate joint swelling.
可可含有具有抗氧化特性的类黄酮。本研究旨在确定可可摄入对佐剂性关节炎等慢性炎症模型相关氧化应激的影响。雌性 Wistar 大鼠喂食 5%或 10%可可强化饮食，或每隔一天给予槲皮素混悬液灌胃 10 天。用加热灭活的分枝杆菌悬液诱导关节炎。测定巨噬细胞产生的活性氧（ROS）以及脾中超氧化物歧化酶（总、胞质和线粒体）和过氧化氢酶的活性。临床上，抗氧化剂并未减轻关节炎大鼠的关节肿胀；然而，5%可可饮食和槲皮素给药减少了 ROS 的产生。此外，5%可可饮食使超氧化物歧化酶和过氧化氢酶的活性正常化。总之，可可饮食可降低与慢性炎症性病理相关的氧化应激，尽管还不足以减轻关节肿胀。
