Song Min-Jung, Lee Seung-Koo, Kim Jong-Hoon, Lim Dae-Soon
Department of Materials Science and Engineering, Korea University, Seoul, Korea.
Anal Sci. 2012;28(6):583-7. doi: 10.2116/analsci.28.583.
A selective dopamine (DA) sensor was developed using gold nanoparticles (Au-NPs) dispersed overoxidized-polyaniline (PANI(ox)) based on a boron-doped diamond (BDD) thin-film electrode. The concentration of the DA was determined using voltammetry as a non-enzymatic sensor. BDD thin film has a high signal-to-noise ratio, a long-term stability, a high sensitivity, and a good reproducibility. PANI nanocomposites were directly synthesized on the BDD electrode and overoxidized using 0.5 M H(2)SO(4) solution. The overoxidized PANI film enhances selectivity and sensitivity toward DA. The Au-NPs were dispersed on the PANI nanocomposite by electrochemical deposition. The nanometer-sized Au-NPs favor the sensing of DA in the presence of ascorbic acid (AA). The combination of the PANI with the Au-NPs and the BDD electrode can create synergetic effects for the performance of the biosensor, such as a fast response time, a lower detection limit, a wider linear range, enhanced selectivity, and higher sensitivity for the determination of DA.
基于硼掺杂金刚石（BDD）薄膜电极，利用分散在过氧化聚苯胺（PANI(ox)）上的金纳米颗粒（Au-NPs）开发了一种选择性多巴胺（DA）传感器。使用伏安法作为非酶传感器测定DA的浓度。BDD薄膜具有高信噪比、长期稳定性、高灵敏度和良好的重现性。聚苯胺纳米复合材料直接在BDD电极上合成，并使用0.5 M H₂SO₄溶液进行过氧化处理。过氧化聚苯胺膜提高了对DA的选择性和灵敏度。通过电化学沉积将Au-NPs分散在聚苯胺纳米复合材料上。纳米尺寸的Au-NPs有利于在抗坏血酸（AA）存在下对DA的传感。聚苯胺与Au-NPs和BDD电极的结合可为生物传感器的性能产生协同效应，如快速响应时间、更低的检测限、更宽的线性范围、增强的选择性以及对DA测定更高的灵敏度。
