Department of Chemistry "Ugo Schiff", University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino (FI), Italy.
Istituto Nazionale Biostrutture e Biosistemi (INBB), Research Unit of Florence, Viale delle Medaglie d'Oro 305, 00136 Roma, Italy.
Sensors (Basel). 2019 Jul 13;19(14):3097. doi: 10.3390/s19143097.
In this work, we report the development of a simple and sensitive sensor based on graphite screen-printed electrodes (GSPEs) modified by a nanocomposite film for dopamine (DA) detection. The sensor was realized by electrodepositing polyaniline (PANI) and gold nanoparticles (AuNPs) onto the graphite working electrode. The sensor surface was fully characterized by means of the cyclic voltammetry (CV) technique using [Fe(CN)] and [Ru(NH)] as redox probes. The electrochemical behavior of the nanocomposite sensor towards DA oxidation was assessed by differential pulse voltammetry (DPV) in phosphate buffer saline at physiological pH. The sensor response was found to be linearly related to DA concentration in the range 1-100 μM DA, with a limit of detection of 0.86 μM. The performance of the sensor in terms of reproducibility and selectivity was also studied. Finally, the sensor was successfully applied for a preliminary DA determination in human serum samples.
在这项工作中，我们报告了一种基于石墨印刷电极（GSPE）的简单灵敏传感器的开发，该传感器通过在石墨工作电极上电沉积聚苯胺（PANI）和金纳米粒子（AuNPs）来实现。通过使用[Fe（CN）]和[Ru（NH）]作为氧化还原探针的循环伏安法（CV）技术对传感器表面进行了全面表征。在生理 pH 值的磷酸盐缓冲盐中通过差分脉冲伏安法（DPV）评估了纳米复合传感器对 DA 氧化的电化学行为。发现传感器对 1-100 μM DA 范围内的 DA 浓度的响应呈线性关系，检测限为 0.86 μM。还研究了传感器在重现性和选择性方面的性能。最后，该传感器成功地应用于人血清样品中 DA 的初步测定。
