Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, College of Life Science, South China Normal University, Guangzhou, People's Republic of China.
Ecotoxicology. 2012 Aug;21(6):1603-8. doi: 10.1007/s10646-012-0947-7. Epub 2012 Jun 23.
White shrimp Litopenaeus vannamei Boone (6-7 cm) were individually exposed during 8 h to 0 (control), 0.5, 1.0, and 10.0 mg/l NO(2)(-)-N at 22 °C and 10 ‰ salinity. Nitrite concentration was measured, and hemolymph and hepatopancreas were sampled at 0, 4 and 8 h. Superoxide dismutase (SOD) activity, catalase (CAT) activity, and malondialdehyde (MDA) in haemolymph and hepatopancreas of L. vannamei Boone, and hemocyanin were examined. The results indicated significant relationships among hemocyanin concentration (Hc), nitrite concentration (nt), and exposure time (t) was as following: Hc = 1.250 + 0.0360 nt + 0.0636t, (R(2) = 0.702, P < 0.01), and Gompertz models with 3-parameters was used to describe well the increase trend of Hc with increase of nitrite concentration at 4, 8 h, respectively (R(2) > 0.99, P ≤ 0.05). MDA level and CAT activity in hemolymph decreased significantly at 8 h, and MDA level and CAT activity in hepatopancreas increased markedly. The SOD activity in hepatopancreas remained almost stably in the range of 0.22-0.24 U/g Hb within 8 h. The results showed there existed a immune difference between in hemolymph and hepatopancreas after white shrimp exposed to ambient nitrite within 8 h, and further experiments should be designed to answer the reason.
白对虾（Litopenaeus vannamei Boone）（6-7 厘米）个体在 22°C 和 10‰盐度下，分别暴露于 8 小时 0（对照）、0.5、1.0 和 10.0mg/L 的 NO(2)(-)-N 中。测量亚硝酸盐浓度，并在 0、4 和 8 小时时取样血淋巴和肝胰腺。检测了血淋巴和肝胰腺中超氧化物歧化酶（SOD）活性、过氧化氢酶（CAT）活性和丙二醛（MDA）以及血蓝蛋白。结果表明，血蓝蛋白浓度（Hc）、亚硝酸盐浓度（nt）和暴露时间（t）之间存在显著关系，如下所示：Hc=1.250+0.0360nt+0.0636t，（R(2)=0.702，P<0.01），并且 4 小时和 8 小时时分别使用三参数 Gompertz 模型很好地描述了 Hc 随亚硝酸盐浓度增加的增加趋势（R(2)>0.99，P≤0.05）。8 小时时血淋巴中的 MDA 水平和 CAT 活性显著降低，肝胰腺中的 MDA 水平和 CAT 活性显著增加。肝胰腺中的 SOD 活性在 8 小时内几乎稳定在 0.22-0.24 U/g Hb 范围内。结果表明，白对虾在 8 小时内暴露于环境亚硝酸盐后，血淋巴和肝胰腺之间存在免疫差异，应进一步设计实验来回答原因。
