Campa-Córdova A I, Hernández-Saavedra N Y, Ascencio F
Marine Pathology Unit, Centre for Biological Research, Apdo Postal 128, La Paz, BCS, 23000, Mexico.
Comp Biochem Physiol C Toxicol Pharmacol. 2002 Dec;133(4):557-65. doi: 10.1016/s1532-0456(02)00125-4.
The immunomodulatory action of superoxide dismutase (SOD) and its possible use as an indicator of immune responses in American white shrimp (Litopenaeus vannamei) were studied. Juvenile shrimp were immersed in aerated beta-glucan and sulfated polysaccharide solutions for 6 h. SOD activity in haemocytes and muscle was quantified to evaluate whether beta-glucan and sulfated polysaccharide induce immunostimulatory activity. Haemocytes and muscle showed similar increased levels of SOD activity (1.5- and 1.4-fold that of control, respectively). Total haemocyte count decreased within the first 24 h after challenge with immunostimulants, but total haemocyte count and total soluble haemocyte protein increased over normal values after 48-120 h. Single immunostimulation with beta-glucan and sulfated polysaccharide is sufficient to generate an increase in the antioxidant activity of L. vannamei SOD.
研究了超氧化物歧化酶（SOD）的免疫调节作用及其作为南美白对虾（凡纳滨对虾）免疫反应指标的潜在用途。将幼虾浸泡在充气的β-葡聚糖和硫酸化多糖溶液中6小时。对血细胞和肌肉中的SOD活性进行定量，以评估β-葡聚糖和硫酸化多糖是否诱导免疫刺激活性。血细胞和肌肉中的SOD活性水平均有相似程度的升高（分别为对照组的1.5倍和1.4倍）。在用免疫刺激剂攻击后的最初24小时内，血细胞总数下降，但在48 - 120小时后，血细胞总数和总可溶性血细胞蛋白超过正常值增加。用β-葡聚糖和硫酸化多糖进行单次免疫刺激足以使凡纳滨对虾SOD的抗氧化活性增加。
