Takegami K T, Siegle R J, Ayers L W
University of Tennessee, Memphis.
J Am Acad Dermatol. 1990 Dec;23(6 Pt 1):1149-52. doi: 10.1016/0190-9622(90)70349-m.
This study used microbiologic counts to evaluate bacterial contamination during cutaneous surgeries of the head and neck. Aerobic bacterial counts of the surgical field in cases with immediate closure (group A, n = 10 patients) and prolonged procedures such as Mohs micrographic surgery (group B, n = 15 patients) were performed three times during the operation. Cultures were taken before antiseptic application, 30 seconds after application, and before closure. Each group showed a statistically significant reduction in aerobic bacteria both 30 seconds after antiseptic application and before closure. All aerobic bacterial counts at both intervals were less than 100,000 colony-forming units/cm2 in each group. The consistent reduction in aerobic bacteria suggests that a clean technique for the two classes of office operating room surgeries provides low-risk conditions for infection in relatively healthy patients.
本研究采用微生物计数法评估头颈部皮肤手术期间的细菌污染情况。在手术过程中,对即时缝合病例(A组,n = 10例患者)和莫氏显微外科手术等延长手术时间的病例(B组,n = 15例患者)的手术区域进行了三次需氧菌计数。在应用防腐剂前、应用后30秒以及缝合前采集培养样本。每组在应用防腐剂后30秒和缝合前需氧菌数量均有统计学意义的显著减少。每组在这两个时间点的所有需氧菌计数均低于100,000菌落形成单位/平方厘米。需氧菌数量的持续减少表明,这两类门诊手术室手术采用的清洁技术为相对健康的患者提供了低感染风险条件。