• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

功能性tonB基因对于流感嗜血杆菌中耳炎的龙猫模型中的毒力和竞争适应性均是必需的。

A functional tonB gene is required for both virulence and competitive fitness in a chinchilla model of Haemophilus influenzae otitis media.

作者信息

Morton Daniel J, Hempel Randy J, Seale Thomas W, Whitby Paul W, Stull Terrence L

机构信息

Department of Pediatrics, University of Oklahoma Health Sciences Center, Oklahoma City, 73104, USA.

出版信息

BMC Res Notes. 2012 Jun 25;5:327. doi: 10.1186/1756-0500-5-327.

DOI:10.1186/1756-0500-5-327
PMID:22731867
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3425130/
Abstract

BACKGROUND

Haemophilus influenzae requires heme for aerobic growth and possesses multiple mechanisms to obtain this essential nutrient.

METHODS

An insertional mutation in tonB was constructed and the impact of the mutation on virulence and fitness in a chinchilla model of otitis media was determined. The tonB insertion mutant strain was significantly impacted in both virulence and fitness as compared to the wildtype strain in this model.

CONCLUSIONS

The tonB gene of H. influenzae is required for the establishment and maintenance of middle ear infection in this chinchilla model of bacterial disease.

摘要

背景

流感嗜血杆菌需血红素进行有氧生长，并拥有多种获取这种必需营养素的机制。

方法

构建了tonB基因的插入突变体，并确定该突变对中耳炎性豚鼠模型中毒力和适应性的影响。在此模型中，与野生型菌株相比，tonB插入突变体菌株在毒力和适应性方面均受到显著影响。

结论

在这种细菌性疾病的豚鼠模型中，流感嗜血杆菌的tonB基因是中耳感染建立和维持所必需的。

相似文献

1
A functional tonB gene is required for both virulence and competitive fitness in a chinchilla model of Haemophilus influenzae otitis media.功能性tonB基因对于流感嗜血杆菌中耳炎的龙猫模型中的毒力和竞争适应性均是必需的。
BMC Res Notes. 2012 Jun 25;5:327. doi: 10.1186/1756-0500-5-327.
2
Phosphorylcholine decreases early inflammation and promotes the establishment of stable biofilm communities of nontypeable Haemophilus influenzae strain 86-028NP in a chinchilla model of otitis media.磷酰胆碱可减轻早期炎症，并在中耳炎的栗鼠模型中促进不可分型流感嗜血杆菌86 - 028NP稳定生物膜群落的形成。
Infect Immun. 2007 Feb;75(2):958-65. doi: 10.1128/IAI.01691-06. Epub 2006 Nov 27.
3
The role of the RNA chaperone Hfq in Haemophilus influenzae pathogenesis.RNA 分子伴侣 Hfq 在流感嗜血杆菌发病机制中的作用。
BMC Microbiol. 2013 Jun 16;13:134. doi: 10.1186/1471-2180-13-134.
4
Reduced severity of middle ear infection caused by nontypeable Haemophilus influenzae lacking the hemoglobin/hemoglobin-haptoglobin binding proteins (Hgp) in a chinchilla model of otitis media.在中耳炎的栗鼠模型中，缺乏血红蛋白/血红蛋白-触珠蛋白结合蛋白（Hgp）的不可分型流感嗜血杆菌引起的中耳感染严重程度降低。
Microb Pathog. 2004 Jan;36(1):25-33. doi: 10.1016/j.micpath.2003.08.007.
5
Virulence phenotypes of low-passage clinical isolates of nontypeable Haemophilus influenzae assessed using the chinchilla laniger model of otitis media.使用青紫蓝兔中耳炎模型评估不可分型流感嗜血杆菌低传代临床分离株的毒力表型。
BMC Microbiol. 2007 Jun 14;7:56. doi: 10.1186/1471-2180-7-56.
6
The heme-binding protein (HbpA) of Haemophilus influenzae as a virulence determinant.流感嗜血杆菌的血红素结合蛋白（HbpA）作为一种毒力决定因素。
Int J Med Microbiol. 2009 Nov;299(7):479-88. doi: 10.1016/j.ijmm.2009.03.004. Epub 2009 May 17.
7
A functional tonB gene is required for both utilization of heme and virulence expression by Haemophilus influenzae type b.功能性tonB基因是b型流感嗜血杆菌利用血红素和表达毒力所必需的。
Infect Immun. 1994 Jun;62(6):2470-7. doi: 10.1128/iai.62.6.2470-2477.1994.
8
Host-derived sialic acid is incorporated into Haemophilus influenzae lipopolysaccharide and is a major virulence factor in experimental otitis media.宿主来源的唾液酸被整合到流感嗜血杆菌的脂多糖中，并且是实验性中耳炎的主要毒力因子。
Proc Natl Acad Sci U S A. 2003 Jul 22;100(15):8898-903. doi: 10.1073/pnas.1432026100. Epub 2003 Jul 10.
9
Comparison of transcription of the Haemophilus influenzae iron/heme modulon genes in vitro and in vivo in the chinchilla middle ear.比较体外和体内豚鼠中耳中流感嗜血杆菌铁/血红素调节基因的转录。
BMC Genomics. 2013 Dec 27;14:925. doi: 10.1186/1471-2164-14-925.
10
Microevolution in response to transient heme-iron restriction enhances intracellular bacterial community development and persistence.对短暂血红素铁限制的微进化增强了细胞内细菌群落的发展和持续存在。
PLoS Pathog. 2018 Oct 17;14(10):e1007355. doi: 10.1371/journal.ppat.1007355. eCollection 2018 Oct.

引用本文的文献

1
Otitis media: recent advances in otitis media vaccine development and model systems.中耳炎：中耳炎疫苗研发及模型系统的最新进展
Front Microbiol. 2024 Jan 24;15:1345027. doi: 10.3389/fmicb.2024.1345027. eCollection 2024.
2
Comparative Genomic Analysis of the Human Pathogen Provides Insight Into the Identification of Antimicrobial Resistance Genotypes and Potential Virulence Traits.对人类病原体的比较基因组分析为鉴定抗菌耐药基因型和潜在毒力特征提供了线索。
Front Cell Infect Microbiol. 2022 Jul 7;12:912427. doi: 10.3389/fcimb.2022.912427. eCollection 2022.
3
Iron Acquisition Systems of Gram-negative Bacterial Pathogens Define TonB-Dependent Pathways to Novel Antibiotics.

本文引用的文献

1
Survival of bacterial biofilms within neutrophil extracellular traps promotes nontypeable Haemophilus influenzae persistence in the chinchilla model for otitis media.中性粒细胞胞外诱捕网内细菌生物膜的存活促进了中耳炎模型中不可分型流感嗜血杆菌的持续存在。
J Innate Immun. 2009;1(3):215-24. doi: 10.1159/000205937. Epub 2009 Feb 27.
2
Characterization of the Haemophilus influenzae tehB gene and its role in virulence.鉴定流感嗜血杆菌 tehB 基因及其在毒力中的作用。
Microbiology (Reading). 2010 Apr;156(Pt 4):1188-1200. doi: 10.1099/mic.0.036400-0. Epub 2010 Jan 14.
3
The heme-binding protein (HbpA) of Haemophilus influenzae as a virulence determinant.
革兰氏阴性细菌病原体的铁获取系统定义了依赖 TonB 的新型抗生素途径。
Chem Rev. 2021 May 12;121(9):5193-5239. doi: 10.1021/acs.chemrev.0c01005. Epub 2021 Mar 16.
4
Antagonistic Pleiotropy in the Bifunctional Surface Protein FadL (OmpP1) during Adaptation of Haemophilus influenzae to Chronic Lung Infection Associated with Chronic Obstructive Pulmonary Disease.双功能表面蛋白 FadL（OmpP1）在流感嗜血杆菌适应与慢性阻塞性肺疾病相关的慢性肺部感染中的拮抗多效性。
mBio. 2018 Sep 25;9(5):e01176-18. doi: 10.1128/mBio.01176-18.
5
Surface Motion of Tympanic Membrane in a Chinchilla Model of Acute Otitis Media.急性中耳炎雪貂模型中鼓膜的表面运动
J Assoc Res Otolaryngol. 2018 Dec;19(6):619-635. doi: 10.1007/s10162-018-00683-2. Epub 2018 Sep 6.
6
Factors affecting sound energy absorbance in acute otitis media model of chinchilla.影响栗鼠急性中耳炎模型中声能吸收的因素。
Hear Res. 2017 Jul;350:22-31. doi: 10.1016/j.heares.2017.04.003. Epub 2017 Apr 10.
7
Dynamic property changes in stapedial annular ligament associated with acute otitis media in the chinchilla.与栗鼠急性中耳炎相关的镫骨环状韧带动态特性变化
Med Eng Phys. 2017 Feb;40:65-74. doi: 10.1016/j.medengphy.2016.12.003. Epub 2016 Dec 15.
8
Morphological changes in the round window membrane associated with Haemophilus influenzae-induced acute otitis media in the chinchilla.与流感嗜血杆菌诱导的栗鼠急性中耳炎相关的圆窗膜形态学变化
Int J Pediatr Otorhinolaryngol. 2016 Sep;88:74-81. doi: 10.1016/j.ijporl.2016.06.049. Epub 2016 Jun 30.
9
From Homodimer to Heterodimer and Back: Elucidating the TonB Energy Transduction Cycle.从同型二聚体到异型二聚体再回归：阐明托品B能量转导循环
J Bacteriol. 2015 Nov;197(21):3433-45. doi: 10.1128/JB.00484-15. Epub 2015 Aug 17.
10
Dynamic Properties of Tympanic Membrane in a Chinchilla Otitis Media Model Measured With Acoustic Loading.用声学负载测量的灰鼠中耳炎模型中鼓膜的动态特性
J Biomech Eng. 2015 Aug;137(8):081006. doi: 10.1115/1.4030410. Epub 2015 Jun 9.
流感嗜血杆菌的血红素结合蛋白（HbpA）作为一种毒力决定因素。
Int J Med Microbiol. 2009 Nov;299(7):479-88. doi: 10.1016/j.ijmm.2009.03.004. Epub 2009 May 17.
4
Nontypeable Haemophilus influenzae as a pathogen in children.不可分型流感嗜血杆菌作为儿童病原体
Pediatr Infect Dis J. 2009 Jan;28(1):43-8. doi: 10.1097/INF.0b013e318184dba2.
5
New substrates for TonB-dependent transport: do we only see the 'tip of the iceberg'?托蛋白B依赖性转运的新底物：我们看到的只是“冰山一角”吗？
Trends Biochem Sci. 2008 Jul;33(7):330-8. doi: 10.1016/j.tibs.2008.04.012. Epub 2008 Jun 6.
6
Lipoprotein e (P4) of Haemophilus influenzae: role in heme utilization and pathogenesis.流感嗜血杆菌的脂蛋白e（P4）：在血红素利用和发病机制中的作用。
Microbes Infect. 2007 Jul;9(8):932-9. doi: 10.1016/j.micinf.2007.03.013. Epub 2007 Apr 5.
7
The haem-haemopexin utilization gene cluster (hxuCBA) as a virulence factor of Haemophilus influenzae.作为流感嗜血杆菌毒力因子的血红素-血红素结合蛋白利用基因簇（hxuCBA）
Microbiology (Reading). 2007 Jan;153(Pt 1):215-224. doi: 10.1099/mic.0.2006/000190-0.
8
Phosphorylcholine decreases early inflammation and promotes the establishment of stable biofilm communities of nontypeable Haemophilus influenzae strain 86-028NP in a chinchilla model of otitis media.磷酰胆碱可减轻早期炎症，并在中耳炎的栗鼠模型中促进不可分型流感嗜血杆菌86 - 028NP稳定生物膜群落的形成。
Infect Immun. 2007 Feb;75(2):958-65. doi: 10.1128/IAI.01691-06. Epub 2006 Nov 27.
9
Complex role of hemoglobin and hemoglobin-haptoglobin binding proteins in Haemophilus influenzae virulence in the infant rat model of invasive infection.血红蛋白和血红蛋白-结合珠蛋白结合蛋白在婴儿大鼠侵袭性感染模型中对流感嗜血杆菌毒力的复杂作用。
Infect Immun. 2006 Nov;74(11):6213-25. doi: 10.1128/IAI.00744-06. Epub 2006 Sep 11.
10
Differential utilization by Haemophilus influenzae of haemoglobin complexed to the three human haptoglobin phenotypes.流感嗜血杆菌对与三种人类触珠蛋白表型复合的血红蛋白的差异利用。
FEMS Immunol Med Microbiol. 2006 Apr;46(3):426-32. doi: 10.1111/j.1574-695X.2006.00052.x.