功能性tonB基因对于流感嗜血杆菌中耳炎的龙猫模型中的毒力和竞争适应性均是必需的。
A functional tonB gene is required for both virulence and competitive fitness in a chinchilla model of Haemophilus influenzae otitis media.
作者信息
Morton Daniel J, Hempel Randy J, Seale Thomas W, Whitby Paul W, Stull Terrence L
机构信息
Department of Pediatrics, University of Oklahoma Health Sciences Center, Oklahoma City, 73104, USA.
出版信息
BMC Res Notes. 2012 Jun 25;5:327. doi: 10.1186/1756-0500-5-327.
BACKGROUND
Haemophilus influenzae requires heme for aerobic growth and possesses multiple mechanisms to obtain this essential nutrient.
METHODS
An insertional mutation in tonB was constructed and the impact of the mutation on virulence and fitness in a chinchilla model of otitis media was determined. The tonB insertion mutant strain was significantly impacted in both virulence and fitness as compared to the wildtype strain in this model.
CONCLUSIONS
The tonB gene of H. influenzae is required for the establishment and maintenance of middle ear infection in this chinchilla model of bacterial disease.
背景
流感嗜血杆菌需血红素进行有氧生长，并拥有多种获取这种必需营养素的机制。
方法
构建了tonB基因的插入突变体，并确定该突变对中耳炎性豚鼠模型中毒力和适应性的影响。在此模型中，与野生型菌株相比，tonB插入突变体菌株在毒力和适应性方面均受到显著影响。
结论
在这种细菌性疾病的豚鼠模型中，流感嗜血杆菌的tonB基因是中耳感染建立和维持所必需的。
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