Instituto Nacional de Recursos Biológicos, I.P./INIA-Dois Portos, Quinta da Almoínha, 2565-191, Dois Portos, Portugal.
Antonie Van Leeuwenhoek. 2012 Nov;102(4):653-8. doi: 10.1007/s10482-012-9762-7. Epub 2012 Jun 26.
Torulopsis bacillaris (Kroemer and Krumbholz) Lodder (basionym Saccharomyces bacillaris Kroemer and Krumbholz) was frequently detected in oenological works on yeast ecology conducted in the mid-1950s in different wine regions of the world, before its unification with Torulopsis stellata (Kroemer and Krumbholz) Lodder. Most of the phenotypic characteristics pointed out for T. bacillaris are currently attributed to Candida zemplinina Sipiczki. In the present work isoenzyme profiles and rDNA restriction profiles of the neotype of S. bacillaris from two yeast culture collections (CBS 843 and PYCC 3044) and of the type strain of C. zemplinina (CBS 9494) were determined and similar profiles were detected. Moreover, the sequences of the D1/D2 region of the 26S rRNA gene of the three strains were 100 % identical. Different profiles were observed for the type strain of C. stellata (CBS 157) both for isoenzyme and rDNA restriction analysis and only 91 % similarity was found between the D1/D2 sequence of this strain and that of the neotype of S. bacillaris. In view of the newly obtained data and the fact that all above-mentioned species belong to the Starmerella clade, only distantly related to Candida tropicalis (the type species of the genus), S. bacillaris is hereby reinstated as Starmerella bacillaris comb. nov., with C. zemplinina as an obligate synonym.
短梗霉（Kroemer 和 Krumbholz）Lodder（原名 Saccharomyces bacillaris Kroemer 和 Krumbholz）在 20 世纪 50 年代中期的不同世界葡萄酒产区的酵母生态学的酒学作品中经常被发现，之后才与酿酒酵母（Kroemer 和 Krumbholz）Lodder 合并。目前，大多数针对短梗霉（Torulopsis bacillaris）的表型特征都归因于念珠菌（Candida zemplinina）Sipiczki。在本研究中，从两个酵母培养物收集物（CBS 843 和 PYCC 3044）的 S. bacillaris 模式种和 C. zemplinina 模式株（CBS 9494）的同工酶图谱和 rDNA 限制图谱进行了测定，并检测到相似的图谱。此外，这三种菌株的 26S rRNA 基因 D1/D2 区的序列完全相同。C. stellata（CBS 157）的模式株在同工酶和 rDNA 限制分析中表现出不同的图谱，并且该菌株与 S. bacillaris 模式种的 D1/D2 序列之间仅发现 91%的相似性。鉴于新获得的数据以及上述所有物种均属于 Starmerella 分支的事实，与热带假丝酵母（属的模式种）关系较远，S. bacillaris 现被重新确立为短梗霉（Starmerella bacillaris），念珠菌（Candida zemplinina）被归为短梗霉（Starmerella bacillaris）的一个强制性同义词。
