正交设计法研究大黄蛰虫丸抗动脉血栓形成的作用

[Study of dahuangzhechong pills on anti-arterial thrombosis with the orthogonal design].

作者信息

Wei Xing, Wang Zhan-Yi, Li Jie-Yang, Wan Ling, He Fu-Yuan, Duan Xiao-Peng, Zhong Guang-Wei, Jiang Yao, Wang Dong-Sheng

机构信息

Xiangya Hospital Central South University, Changsha 410008, China.

出版信息

Zhong Yao Cai. 2012 Jan;35(1):106-11.

PMID:22734422

Abstract

OBJECTIVE

To screen the main component of Dahuangzhechong pill's anti-arterial thrombosis with the orthogonal design and refine Dahuangzhechong pills.

METHODS

In accordance with the orthogonal design table (L(16)2(15)), divided herbs into 16 groups and made the appropriate liquid. The liquid was gave to SD rats by intragastric administration,the model group, normal control group received the same volume of physiological saline. Isolated rats' carotid artery after intragastric administration a week,modeled according to ferric chloride inducement the carotid artery thrombosis method, then collected blood, detected content of platelet, thromboxane B2 (TXB2) and 6-keto-prostaglandin F1alpha (6-keto-PGF1alpha), sheared and measured dry weight of the modeling artery, then placed arteries in 10% formalin fixation, observed morphological changes in vascular tissue by HE staining.

RESULTS

Pathological examination revealed: each experimental group had thrombosis, softening, dissolution, absorption, and intimal injury, but the severity of thrombosis were diferent. Orthogonal analysis showed: 1, influence on dry weight of thrombus: rhubarb, ground beetle, leeches, peach seed, dry paint, except dry paint P<0.05, the others P<0.01.2, influence on plasma 6- keto-PGF1alpha level: peach seed, dry paint, ground beetle, gadfly, grubs, leeches, rhubarb, except rhubarb P<0.05, the others P<0.01.3, influence on plasma TXB2: ground beetle, peach seed, dried paint, rhubarb, leeches, except leech P<0.05, the others P<0.01.4, influence on platelet count: peach seed, dry paint, rhubarb, ground beetle, gadfly, leeches, except gadfly, leeches P<0.05, the others P<0.01.

CONCLUSION

Anti-artery thrombosis of Dahuangzhechong Pill is most closely related with rhubarb, ground beetle, leeches, peach seed, dry paint and gadfly.

摘要

目的

采用正交设计筛选大黄蛰虫丸抗动脉血栓形成的主要成分，并对大黄蛰虫丸进行优化。

方法

按照正交设计表（L(16)2(15)），将药材分为16组并制成相应的药液。将药液灌胃给予SD大鼠，模型组、正常对照组给予等体积生理盐水。灌胃一周后分离大鼠颈动脉，按照氯化铁诱导颈动脉血栓形成法造模，然后取血，检测血小板、血栓素B2（TXB2）和6-酮-前列腺素F1α（6-酮-PGF1α）含量，剪下并测量造模动脉的干重，然后将动脉置于10%福尔马林中固定，通过HE染色观察血管组织的形态变化。

结果

病理检查显示：各实验组均有血栓形成、软化、溶解、吸收及内膜损伤，但血栓形成的严重程度不同。正交分析显示：1. 对血栓干重的影响：大黄、土鳖虫、水蛭、桃仁、干漆，除干漆P<0.05外，其余P<0.01。2. 对血浆6-酮-PGF1α水平的影响：桃仁、干漆、土鳖虫、虻虫、蛴螬、水蛭、大黄，除大黄P<0.05外，其余P<0.01。3. 对血浆TXB2的影响：土鳖虫、桃仁、干漆、大黄、水蛭，除水蛭P<0.05外，其余P<0.01。4. 对血小板计数的影响：桃仁、干漆、大黄、土鳖虫、虻虫、水蛭，除虻虫、水蛭P<0.05外，其余P<0.01。