乙酰氨基部分甲基的1H核磁共振谱及溶液中酸性糖胺聚糖的结构
1H nuclear-magnetic-resonance spectra of the methyl group of the acetamido moiety and the structure of acid glycosaminoglycans in solution.
作者信息
Scott J E, Heatley F
出版信息
Biochem J. 1979 Aug 1;181(2):445-9. doi: 10.1042/bj1810445.
Abstract
The 1H resonances of the methyl group in the acetamido moiety of several types of glycosaminoglycans are reported at 300 MHz in 2H2O. Dermatan sulphates with various L-iduronate/D-glucuronate ratios are compared with chrondroitin sulphates with various contents and positions of substitution of sulphate esters. Hyaluronate oligomers are compared with 2-acetamido-2-deoxy-D-glucose, and with heparan sulphate and keratan sulphate. The major determinant of the chemical shift of the acetamido methyl resonance is the closeness of approach between carboxy groups and the acetamido group, in agreement with chemical evidence based on periodate-oxidation kinetics.
摘要
在2H2O中于300兆赫下报道了几种类型糖胺聚糖的乙酰氨基部分中甲基的1H共振。将具有不同L-艾杜糖醛酸/D-葡萄糖醛酸比例的硫酸皮肤素与具有不同硫酸酯取代含量和位置的硫酸软骨素进行比较。将透明质酸寡聚物与2-乙酰氨基-2-脱氧-D-葡萄糖、硫酸乙酰肝素和硫酸角质素进行比较。乙酰氨基甲基共振化学位移的主要决定因素是羧基与乙酰氨基之间的接近程度，这与基于高碘酸盐氧化动力学的化学证据一致。
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