水分子参与透明质酸的二级结构。
A water molecule participates in the secondary structure of hyaluronan.
作者信息
Heatley F, Scott J E
机构信息
Department of Chemistry, University of Manchester, U.K.
出版信息
Biochem J. 1988 Sep 1;254(2):489-93. doi: 10.1042/bj2540489.
Abstract
The structure of hyaluronan was investigated in water/dimethyl sulphoxide mixtures by using high-field n.m.r. and space-filling molecular models. The secondary structure previously established in detail in 'dry' dimethyl sulphoxide [Heatley, Scott & Hull (1984) Biochem. J. 220, 197-205] undergoes changes on addition of water, compatible with the incorporation of a water bridge between the uronate carboxylate and acetamido NH groups. Molecular models show that such a configuration is highly probable, and saturation-transfer experiments yield rates of NH proton exchange that support this proposed structure. The existence of two distinct stable configurations for hyaluronan, in water-rich and water-poor conditions respectively, may have biological implications, e.g. during its biosynthesis in cell membranes. There are extensive hydrophobic regions in both forms, which may be important for interactions with e.g., membranes, proteins and itself.
摘要
通过使用高场核磁共振和空间填充分子模型，在水/二甲基亚砜混合物中研究了透明质酸的结构。先前在“干燥”二甲基亚砜中详细确定的二级结构[希特利、斯科特和赫尔（1984年）《生物化学杂志》220卷，197 - 205页]在加入水后会发生变化，这与在糖醛酸羧酸盐和乙酰氨基NH基团之间形成水桥相一致。分子模型表明这种构型极有可能，饱和转移实验得出的NH质子交换速率支持了这一提出的结构。透明质酸分别在富水和贫水条件下存在两种不同的稳定构型，这可能具有生物学意义，例如在其于细胞膜中的生物合成过程中。两种形式都有广泛的疏水区域，这对于与例如膜、蛋白质及其自身的相互作用可能很重要。
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