Bokaeian M, Khazaei H A, Javadimehr M
Department of Laboratory Sciences, School of Paramedical Sciences, Zahedan University of Medical Sciences, Zahedan, Iran.
Iran Red Crescent Med J. 2011 May;13(5):328-33. Epub 2011 May 1.
Colonization of nasopharynx by Streptococcus pneumoniae can lead to pneumococcal diseases. This study was performed to determine the carriage rate of nasopharyngeal S. pneumoniae in adolescents, antibiotic susceptibility and serotype prevalence in Zahedan, Iran.
Nasopharyngeal specimens from 865 adolescents (age range: 10-19 years old) attending eight schools in Zahedan, Iran, were collected and assessed by standard procedures to recover S. pneumoniae. The serotyping was carried out by latex agglutination test and the minimum inhibitory concentrations (MIC) of penicillin as well as other commonly used antibiotics were determined by a broth dilution method.
Pneumococci were recovered from 15.7% (136/865, 95% confidence interval (CI) 12.3-18.9) of total samples which 119 isolates were typable with the available antisera. 1, 19A, 15C, 9V, 11A and 19F were found as the most frequent serotypes. Ninety three pneumococcal isolates were sensitive to penicillin. The MIC values of antibiotics tested were (μg/ml): penicillin 0.01-4, cefotaxime 0.01-4, ceftriaxone 0.02-128, chloramphenicol 0.08-32, ciprofloxacin 0.06-16, erythromycin 0.01-128, tetracycline 0.08-128 and vancomycin 0.02-1.
A clear diversity was seen in the serotype distribution of the S. pneumoniae isolates and most of the antibiotic resistant strains belonged to few serotypes. Healthy adolescents in Zahedan, Iran commonly show pneumococcal carriage and antibiotic resistance.
肺炎链球菌在鼻咽部定植可导致肺炎球菌性疾病。本研究旨在确定伊朗扎黑丹青少年鼻咽部肺炎链球菌的携带率、抗生素敏感性及血清型流行情况。
收集了伊朗扎黑丹8所学校865名青少年（年龄范围：10 - 19岁）的鼻咽部标本，采用标准程序进行检测以分离肺炎链球菌。通过乳胶凝集试验进行血清分型，采用肉汤稀释法测定青霉素以及其他常用抗生素的最低抑菌浓度（MIC）。
从15.7%（136/865，95%置信区间（CI）12.3 - 18.9）的样本中分离出肺炎球菌，其中119株分离株可用现有抗血清进行分型。发现1、19A、15C、9V、11A和19F为最常见的血清型。93株肺炎球菌分离株对青霉素敏感。所检测抗生素的MIC值（μg/ml）分别为：青霉素0.01 - 4、头孢噻肟0.01 - 4、头孢曲松0.02 - 128、氯霉素0.08 - 32、环丙沙星0.06 - 16、红霉素0.01 - 128、四环素0.08 - 128、万古霉素0.02 - 1。
肺炎链球菌分离株的血清型分布存在明显差异，大多数耐药菌株属于少数几种血清型。伊朗扎黑丹的健康青少年普遍存在肺炎球菌携带及抗生素耐药情况。
