Division of Plant Pathology, Taiwan Agricultural Research Institute , Council of Agriculture, 189 Zhongzheng Road, Wufeng District, Taichung 41362, Taiwan.
J Agric Food Chem. 2012 Aug 1;60(30):7341-4. doi: 10.1021/jf301570y. Epub 2012 Jul 17.
Secondary metabolites of Clitocybe nuda displayed antimicrobial activity against Phytophthora capsici. The culture filtrate of C. nuda was extracted with ethanol and chromatographically separated on a Sephadex LH-20 column and fractionated on a silica gel column to give eight fractions. These fractions were tested for the ability to inhibit zoospore germination of P. capsici . The most active fraction was further purified by silica gel column chromatography to yield three compounds: 2-methoxy-5-methyl-6-methoxymethyl-p-benzoquinone (1), 6-hydroxy-2H-pyran-3-carbaldehyde (2), and indole-3-carbaldehyde (3), all new to C. nuda. At a concentration of 500 mg/L, compound 3 showed complete inhibition of zoospore germination, while compounds 1 and 2 showed inhibition rates of 97 and 86%, respectively. To our knowledge, compound 1 is a newly discovered compound and, for the other two compounds, this is the first report in C. nuda. These compounds are potential candidates for new edible fungi-derived pesticides for the control of plant diseases.
糙皮侧耳（Clitocybe nuda）的次生代谢产物对辣椒疫霉（Phytophthora capsici）显示出抗菌活性。用乙醇提取糙皮侧耳（C. nuda）的培养液，用 Sephadex LH-20 柱进行色谱分离，并在硅胶柱上进行馏分，得到 8 个馏分。这些馏分被测试抑制辣椒疫霉菌游动孢子萌发的能力。最活跃的馏分通过硅胶柱色谱法进一步纯化，得到三种化合物：2-甲氧基-5-甲基-6-甲氧基甲基对苯醌（1）、6-羟基-2H-吡喃-3-甲醛（2）和吲哚-3-甲醛（3），所有这些在糙皮侧耳（C. nuda）中均为新化合物。在 500mg/L 浓度下，化合物 3 完全抑制游动孢子的萌发，而化合物 1 和 2 的抑制率分别为 97%和 86%。据我们所知，化合物 1 是一种新发现的化合物，而对于其他两种化合物，这是在糙皮侧耳（C. nuda）中的首次报道。这些化合物是用于防治植物病害的新型可食用真菌衍生农药的潜在候选物。
