辣椒植株上由[未提及具体病菌名称]引起的病害的生物防治。

Biocontrol of Diseases Caused by and in Pepper Plants.

作者信息

Santos Mila, Diánez Fernando, Sánchez-Montesinos Brenda, Huertas Victoria, Moreno-Gavira Alejandro, Esteban García Belén, Garrido-Cárdenas José A, Gea Francisco J

机构信息

Departamento de Agronomía, Escuela Superior de Ingeniería, Universidad de Almería, 04120 Almería, Spain.

Departamento de Agronomía, División Ciencias de la Vida, Campus Irapuato-Salamanca, Universidad de Guanajuato, Irapuato 36500, Guanajuato, Mexico.

出版信息

J Fungi (Basel). 2023 Mar 15;9(3):360. doi: 10.3390/jof9030360.

The main objective of this study was to evaluate the ability of f. , , , and as biological control agents (BCAs) against diseases caused by and in pepper. For this purpose, their antagonistic activities were evaluated both in vitro and in vivo. We analysed the expression patterns of five defence related genes, , , , , and , in leaves. All BCAs showed a high in vitro antagonistic activity, significantly reducing the mycelial growth of and . The treatments with f. , , and substantially reduced the severity of the disease caused by by 54, 76, and 70%, respectively, and of the disease caused by by 66, 55, and 64%, respectively. had the lowest values of disease reduction. Reinoculation with the four BCAs increased the control of both plant pathogens. Markedly different expression patterns were observed in the genes , , and . Based on the results, all four BCAs under study could be used as a biological alternative to chemicals for the control of and in pepper with a high success rate.

本研究的主要目的是评估哈茨木霉、绿色木霉、枯草芽孢杆菌和多粘类芽孢杆菌作为生物防治剂（BCAs）对辣椒疫霉和炭疽病菌引起的病害的防治能力。为此，对它们的拮抗活性进行了体外和体内评估。我们分析了叶片中五个与防御相关的基因，即病程相关蛋白基因、几丁质酶基因、β-1,3-葡聚糖酶基因、过氧化物酶基因和苯丙氨酸解氨酶基因的表达模式。所有生物防治剂均表现出较高的体外拮抗活性，显著降低了辣椒疫霉和炭疽病菌的菌丝生长。哈茨木霉、绿色木霉和枯草芽孢杆菌处理分别使辣椒疫霉引起的病害严重程度大幅降低了54%、76%和70%，使炭疽病菌引起的病害严重程度分别降低了66%、55%和64%。多粘类芽孢杆菌的病害降低值最低。用这四种生物防治剂重新接种提高了对两种植物病原菌的防治效果。在病程相关蛋白基因、几丁质酶基因和β-1,3-葡聚糖酶基因中观察到明显不同的表达模式。基于这些结果，所研究的四种生物防治剂都可以作为化学药剂的生物替代品，用于高效防治辣椒疫霉和炭疽病菌。