Lee Jin Seok, Bae Min Ho, Choi Sung Ho, Lee Sang Hun, Cho Young Sam, Park Heung Jae, Kwon Chil Hun, Joo Kwan Joong
Department of Urology, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Korea.
Korean J Urol. 2012 Jun;53(6):396-400. doi: 10.4111/kju.2012.53.6.396. Epub 2012 Jun 19.
Animal tumor models are important for the evaluation of novel therapeutic modalities. Since the initial report of an orthotopic bladder tumor model, several modifications have been proposed to improve the tumor take rate. Here we compared the HCl-pretreated and electrocauterization-pretreated orthotopic murine bladder tumor models.
MBT-2 murine bladder cancer cells were transurethrally implanted in the bladder of syngeneic C3H/He mice. The mice were divided into three groups according to pretreatment methods (electrocautery, HCl, and control group) and were subjected to pretreatment before instillation of MBT-2 tumor cells into the bladder. Mice were sacrificed on day 21, and bladders were harvested, weighed, and examined histopathologically.
The tumor take rate of the control, electrocautery, and HCl groups was 0%, 54%, and 100%, respectively. The tumor take rate of the HCl group was significantly higher than that of the control group (p<0.01) and the electrocautery group (p=0.01). Pathologic reports revealed that all established bladder tumors were high-grade papillary urothelial carcinomas.
The HCl pretreatment model was a preferable murine bladder tumor model for evaluating further therapeutic interventions.
动物肿瘤模型对于评估新型治疗方式很重要。自从原位膀胱肿瘤模型首次报道以来，已提出了几种改进方法以提高肿瘤接种成功率。在此，我们比较了盐酸预处理和电灼预处理的原位小鼠膀胱肿瘤模型。
将MBT-2小鼠膀胱癌细胞经尿道植入同基因C3H/He小鼠的膀胱。根据预处理方法（电灼、盐酸和对照组）将小鼠分为三组，并在将MBT-2肿瘤细胞注入膀胱之前进行预处理。在第21天处死小鼠，取出膀胱，称重，并进行组织病理学检查。
对照组、电灼组和盐酸组的肿瘤接种成功率分别为0%、54%和100%。盐酸组的肿瘤接种成功率显著高于对照组（p<0.01）和电灼组（p=0.01）。病理报告显示，所有已形成的膀胱肿瘤均为高级别乳头状尿路上皮癌。
盐酸预处理模型是用于评估进一步治疗干预措施的更优小鼠膀胱肿瘤模型。
