Laboratory of Rickettsiae, Chlamydiae, and Enzotic Spirochetes, National Institute of Public Heath-National Institute of Hygiene, Warsaw, Poland.
Vector Borne Zoonotic Dis. 2011 Jul;11(7):985-9. doi: 10.1089/vbz.2010.0080. Epub 2010 Nov 17.
The presence of antibodies to Rickettsia conorii, R. helvetica, R. felis, R. slovaca, R. sibirica, and R. massiliae in sera of 129 forest workers from northeastern and southern Poland was assayed by indirect immunofluorescence. Previous environmental studies revealed presence of spotted fever group (SFG) rickettsiae in ticks collected from these areas. Additionally, the workers were examinated for the presence of antibodies specific to other tick-borne bacteria: Anaplasma phagocytophilum, Bartonella spp., and B. burgdorferi. The results of the studies have shown the presence of specific SFG rickettsiae antibodies in 14.7% of tested forest workers, among them 78.9% had species-specific antibodies to R. massiliae. Contrary to previous detection R. helvetica and R. slovaca in ticks collected in the environment of the examined area, no species-specific antibodies to these species were detected in studied workers. Antibodies to B. burgdorferi (44%) were found in forest workers more often than antibodies to other tested pathogens. B. burgdorferi was also the main component of coinfections. The most frequent confirmed serologically coinfections were simultaneous infections with B. burgdorferi and Bartonella spp. found in 10% of tested individuals. So far, SFG rickettsiae infections have not been diagnosed in Poland; however, the presence of the bacteria in ticks and presence of specific antibodies in humans exposed to arthropods show the need for monitoring the situation. The list of tick-borne pathogens is increasing, but knowledge about the possibility of humans acquiring multipathogens infections after tick bite still needs evaluation.
采用间接免疫荧光法检测了来自波兰东北部和南部的 129 名森林工作者血清中抗恙虫病东方体、立氏立克次体、猫立克次体、斯洛伐克立克次体、西伯利亚立克次体和马赛立克次体抗体的存在。先前的环境研究表明，在这些地区采集的蜱中存在斑点热群（SFG）立克次体。此外，还检查了这些工人是否存在针对其他蜱传细菌的抗体：嗜吞噬细胞无形体、巴尔通体属和伯氏疏螺旋体。研究结果表明，在所测试的森林工作者中，有 14.7%存在特定的 SFG 立克次体抗体，其中 78.9%对马赛立克次体具有种特异性抗体。与先前在检查区域环境中采集的蜱中检测到立氏立克次体和斯洛伐克立克次体不同，在研究的工人中未检测到针对这些物种的种特异性抗体。在森林工作者中发现针对伯氏疏螺旋体的抗体（44%）比针对其他测试病原体的抗体更常见。伯氏疏螺旋体也是合并感染的主要成分。最常见的经血清学确认的合并感染是同时感染伯氏疏螺旋体和巴尔通体属，在 10%的受检个体中发现。到目前为止，在波兰尚未诊断出 SFG 立克次体感染；然而，蜱中的细菌存在以及暴露于节肢动物的人类中存在特定抗体表明需要监测这种情况。蜱传病原体的清单在不断增加，但关于人类在被蜱叮咬后获得多病原体感染的可能性的知识仍需评估。
