Department of Anesthesiology, Laboratory of Experimental Anesthesiology, Erasmus MC—University Medical Center Rotterdam, Rotterdam, The Netherlands.
Opt Lett. 2012 Jul 1;37(13):2625-7. doi: 10.1364/OL.37.002625.
Mitochondrial oxygen tension can be measured in vivo by means of oxygen-dependent quenching of delayed fluorescence of protoporphyrin IX (PpIX). Here we demonstrate that mitochondrial PO(2) (mitoPO(2)) can be measured in the skin of a rat after topical application of the PpIX precursor 5-aminolevulinic acid (ALA). Calibration of mitoPO(2) measurements was done by comparison with simultaneous measurements of the cutaneous microvascular PO(2) This was done under three different conditions: in normal skin tissue, in nonrespiration skin tissue due to the application of cyanide, and in anoxic skin tissue after the ventilation with 100% nitrogen. The results of this study show that it is feasible to measure the mitoPO(2) after the topical application of ALA cream by means of the PpIX-triplet state lifetime technique.
线粒体氧张力可以通过依赖氧的原卟啉 IX（PpIX）延迟荧光猝灭的方法在体内进行测量。在这里，我们证明了在大鼠皮肤表面涂抹 PpIX 前体 5-氨基酮戊酸（ALA）后，可以测量线粒体 PO2（mitoPO2）。通过与同时测量皮肤微血管 PO2 的方法进行比较，对 mitoPO2 的测量进行了校准。这是在三种不同条件下进行的：在正常皮肤组织中、由于应用氰化物导致的非呼吸性皮肤组织中和在通气后用 100%氮气导致的缺氧性皮肤组织中。这项研究的结果表明，通过 PpIX 三重态寿命技术，在 ALA 乳膏的局部应用后测量 mitoPO2 是可行的。
