Department of Anesthesiology, Laboratory of Experimental Anesthesiology, ErasmusMC, University Medical Center Rotterdam, Rotterdam, The Netherlands.
J Biophotonics. 2011 Oct;4(10):731-9. doi: 10.1002/jbio.201100040. Epub 2011 Jul 19.
Mitochondrial oxygen tension can be measured in vivo by means of oxygen-dependent quenching of delayed fluorescence of protoporphyrin IX (PpIX). Here we demonstrate that delayed fluorescence is readily observed from skin in rat and man after topical application of the PpIX precursor 5-aminolevulinic acid (ALA). Delayed fluorescence lifetimes respond to changes in inspired oxygen fraction and blood supply. The signals contain lifetime distributions and the fitting of rectangular distributions to the data appears more adequate than mono-exponential fitting. The use of topically applied ALA for delayed fluorescence lifetime measurements might pave the way for clinical use of this technique.
线粒体氧张力可以通过依赖氧的原卟啉 IX（PpIX）延迟荧光猝灭的方法在体测量。在这里，我们证明了在大鼠和人中，经皮应用原卟啉 IX 前体 5-氨基酮戊酸（ALA）后，皮肤很容易观察到延迟荧光。延迟荧光寿命对吸入氧分数和血液供应的变化有反应。信号包含寿命分布，用矩形分布对数据进行拟合比单指数拟合更合适。使用局部应用的 ALA 进行延迟荧光寿命测量可能为该技术的临床应用铺平道路。
