Laboratory for Optical and Computational Instrumentation, University of Wisconsin at Madison, Madison, Wisconsin, USA.
Nat Methods. 2012 Jun 28;9(7):697-710. doi: 10.1038/nmeth.2084.
Few technologies are more widespread in modern biological laboratories than imaging. Recent advances in optical technologies and instrumentation are providing hitherto unimagined capabilities. Almost all these advances have required the development of software to enable the acquisition, management, analysis and visualization of the imaging data. We review each computational step that biologists encounter when dealing with digital images, the inherent challenges and the overall status of available software for bioimage informatics, focusing on open-source options.
在现代生物实验室中，成像技术比其他技术更为普及。光学技术和仪器的最新进展正在提供以前无法想象的功能。几乎所有这些进展都需要开发软件来实现对成像数据的采集、管理、分析和可视化。我们回顾了生物学家在处理数字图像时遇到的每个计算步骤、固有的挑战以及生物图像信息学中可用软件的整体状况，重点介绍了开源选项。
