Department of Biomedical Engineering, Tsinghua University School of Medicine, Beijing 100084, China.
Analyst. 2012 Aug 21;137(16):3760-6. doi: 10.1039/c2an35410a. Epub 2012 Jun 29.
Multiplexed analysis allows researchers to obtain high-density information with minimal assay time, sample volume and cost. Currently, microcarrier or particle-based approaches for multiplexed analysis involve complicated or expensive encoding and decoding processes. In this paper, a novel optical encoding technique based on nano-silicon dioxide film is presented. Microcarriers composed of thermally grown silicon dioxide (SiO(2)) film and monocrystalline silicon (Si) substrate were fabricated. The nano-silicon dioxide film exhibited unique surface color by low-coherence interference. Hence the colors can be used for encoding at least 100 microcarriers loaded with films of different thickness. We demonstrated that color-encoded microcarriers loaded with antigens could be used for multiplexed immunoassays to detect goat anti-human IgG, goat anti-mouse IgG and goat anti-rabbit IgG, with fluorescent detection as the interrogating approach. This microcarrier-based method also exhibited improved analytical performance compared with a microarray technique. This approach will provide new opportunities for multiplexed target assay development.
多重分析允许研究人员在最小的分析时间、样品体积和成本下获得高密度的信息。目前，基于微载体或粒子的多重分析方法涉及复杂或昂贵的编码和解码过程。本文提出了一种基于纳米二氧化硅薄膜的新型光学编码技术。由热生长二氧化硅（SiO2）薄膜和单晶硅（Si）衬底组成的微载体。纳米二氧化硅薄膜通过低相干干涉显示出独特的表面颜色。因此，颜色可用于对至少 100 个装载有不同厚度薄膜的微载体进行编码。我们证明，装载有抗原的彩色编码微载体可以用于多重免疫分析，以荧光检测作为检测方法来检测山羊抗人 IgG、山羊抗鼠 IgG 和山羊抗兔 IgG。与微阵列技术相比，这种基于微载体的方法也表现出了更好的分析性能。这种方法将为多重目标分析的发展提供新的机会。
