Hammad Huda M, Nagrash Omar M, Safadi Rima A
Department of Oral Medicine and Oral Surgery, Faculty of Dentistry, Jordan University of Science and Technology, P. O. Box 3030, Irbid 22110, Jordan.
Al-Ramtha Governmental Hospital, Ministry of Health, P. O. Box 132, Al-Ramtha 21410, Jordan.
Int J Dent. 2020 Jul 14;2020:7041520. doi: 10.1155/2020/7041520. eCollection 2020.
The odontogenic keratocyst (OKC) is a controversial lesion that was reclassified as a tumor with the name "keratocystic odontogenic tumor" in 2005. The reclassification was revoked recently in 2017, with a conclusion on the need for further studies on the subject. In this study, the expressions of an important regulatory protein (maspin), an important integral membrane proteoglycan (syndecan-1), and a universal proliferation marker (Ki-67) in the epithelium of the OKC were investigated in comparison with the dentigerous cyst (DC) and ameloblastoma (AB). Twenty-six OKCs, eleven DCs, and ten conventional ABs were immunohistochemically stained for maspin, syndecan-1, and Ki-67. ImageJ was used to analyze the positivity of maspin and syndecan-1. The Ki-67 score was calculated as the percentage of positive nuclei in 5 high power fields. Analysis of variance (ANOVA) test and Student -test were used as appropriate. Lower expressions of maspin were noted in OKC and DC compared to those in AB, and lower expressions of syndecan-1 were noted in OKC and AB compared to those in DC. The differences, however, did not reach statistical significance (ANOVA and -test: > 0.05). The Ki-67 score was significantly higher in OKC than in DC (-test: < 0.05), and not significantly different from AB (-test: > 0.05). In conclusion, expressions of maspin and syndecan-1 are not strongly representative of differences in behavior between OKC, AB, and DC. However, the expression of Ki-67 indicates comparable proliferative activities of OKC and AB, which are higher than that of DC. Further investigation on the biologic behavior of OKC is still recommended to arrive at more specific conclusions regarding its classification.
牙源性角化囊肿（OKC）是一种存在争议的病变，2005年它被重新归类为肿瘤，命名为“角化囊性牙源性肿瘤”。最近在2017年这一重新分类被撤销，得出了需要对该主题进行进一步研究的结论。在本研究中，与含牙囊肿（DC）和成釉细胞瘤（AB）相比，研究了OKC上皮中一种重要的调节蛋白（maspin）、一种重要的整合膜蛋白聚糖（syndecan - 1）和一种通用增殖标志物（Ki - 67）的表达情况。对26个OKC、11个DC和10个传统AB进行了maspin、syndecan - 1和Ki - 67的免疫组织化学染色。使用ImageJ分析maspin和syndecan - 1的阳性情况。Ki - 67评分计算为5个高倍视野中阳性细胞核的百分比。酌情使用方差分析（ANOVA）检验和学生t检验。与AB相比，OKC和DC中maspin的表达较低，与DC相比，OKC和AB中syndecan - 1的表达较低。然而，这些差异未达到统计学意义（ANOVA和t检验：P>0.05）。OKC中的Ki - 67评分显著高于DC（t检验：P<0.05），与AB无显著差异（t检验：P>0.05）。总之，maspin和syndecan - 1的表达并不能强烈代表OKC、AB和DC之间行为的差异。然而，Ki - 67的表达表明OKC和AB具有相当的增殖活性，高于DC。仍建议对OKC的生物学行为进行进一步研究，以得出关于其分类的更具体结论。
