Institute of Biology, Department of Microbiology, Jan Kochanowski University, 25-406, Kielce, Poland.
Clin Biochem. 2012 Nov;45(16-17):1374-82. doi: 10.1016/j.clinbiochem.2012.06.021. Epub 2012 Jun 27.
Proteus mirabilis strains are human pathogens responsible for urinary tract infections, which may also be involved in rheumatoid arthritis (RA).
We determined whether the binding site of anti-LPS antibodies on the O-polysaccharide part of P. mirabilis LPS correlates with the level of TLR4 (Thr399Ile) gene polymorphism in the sera of RA patients. We investigated the deposition of C3d and C5b complement components on the P. mirabilis LPS. The ELISA method used in this study was optimized with LAL test and laser interferometry.
Depending on LPS P. mirabilis used in these studies, the amount of antibodies in RA patients sera varied. We did not observe a correlation between anti-LPS antibodies binding and the level of TLR4 (Thr399Ile) gene polymorphism. We found that the lower complement components deposition by O49 in contrast to O9 LPS correlates with its reduced sensitivities to human complement-mediated killing.
The immunological response against P. mirabilis LPS might play a role in rheumatoid arthritis.
奇异变形杆菌菌株是引起尿路感染的人类病原体，也可能与类风湿关节炎（RA）有关。
我们确定了抗 LPS 抗体在奇异变形杆菌 LPS 的 O-多糖部分上的结合位点是否与 RA 患者血清中 TLR4（Thr399Ile）基因多态性的水平相关。我们研究了 C3d 和 C5b 补体成分在奇异变形杆菌 LPS 上的沉积。本研究中使用的 ELISA 方法通过 LAL 测试和激光干涉法进行了优化。
根据用于这些研究的 LPS 奇异变形杆菌，RA 患者血清中的抗体量有所不同。我们没有观察到抗 LPS 抗体结合与 TLR4（Thr399Ile）基因多态性水平之间的相关性。我们发现 O49 与 O9 LPS 相比，补体成分的沉积量较低，这与其对人补体介导杀伤的敏感性降低有关。
针对奇异变形杆菌 LPS 的免疫反应可能在类风湿关节炎中起作用。
