Department of Pathology, the Third Affiliated (Tumour) Hospital, Harbin Medical University, Harbin 150040, China.
Int J Biol Macromol. 2012 Dec;51(5):968-73. doi: 10.1016/j.ijbiomac.2012.06.031. Epub 2012 Jun 30.
In this study, we purified a homogeneous polysaccharide (PGPW1) from the root of Panax ginseng. Its molecular weight was estimated to be 3.5×10(5) Da by high performance liquid chromatography (HPLC) and Gas chromatography (GC) analysis identified that PGPW1 contained Glc, Gal, Man and Ara in the molar ratio of 3.3:1.2:0.5:1.1. Furthermore the antitumor potential of PGPW1 on human bladder T24 cells was evaluated in vitro by MTT, lactate dehydrogenase (LDH), wound scratch and transwell motility assays. PGPW1 dose-dependently displayed potent anti-proliferation and anti-metastatic activities. Moreover the modulating effect of PGPW1 on the binding of (3)H-NMS to M3 muscarinic receptors on the surface of T24 cells was evaluated. In muscarinic receptor binding assay, the attenuated expression of M3 muscarinic receptor on the surface of T24 cells by PGPW1 would contribute to its antitumor functions. All the data indicated the potential of its clinical application for the prevention and treatment of bladder cancer metastasis.
在这项研究中，我们从人参根部分离得到了一种均一的多糖（PGPW1）。通过高效液相色谱（HPLC）和气相色谱（GC）分析，估计其分子量为 3.5×10(5) Da。GC 分析鉴定 PGPW1 含有 Glc、Gal、Man 和 Ara，摩尔比为 3.3:1.2:0.5:1.1。此外，通过 MTT、乳酸脱氢酶（LDH）、划痕和 Transwell 迁移实验，在体外评估了 PGPW1 对人膀胱 T24 细胞的抗肿瘤潜力。PGPW1 呈剂量依赖性显示出强大的抗增殖和抗转移活性。此外，还评估了 PGPW1 对（3）H-NMS 与人膀胱 T24 细胞表面 M3 毒蕈碱受体结合的调节作用。在毒蕈碱受体结合实验中，PGPW1 对 T24 细胞表面 M3 毒蕈碱受体的表达减弱，有助于其抗肿瘤功能。所有数据表明，其在预防和治疗膀胱癌转移方面具有临床应用的潜力。
